1. Stem Cells and Regenerative Medicine
  2. Chromosomes and Gene Expression
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MOF-associated complexes have overlapping and unique roles in regulating pluripotency in embryonic stem cells and during differentiation

  1. Sarina Ravens
  2. Marjorie Fournier
  3. Tao Ye
  4. Matthieu Stierle
  5. Doulaye Dembele
  6. Virginie Chavant
  7. Làszlò Tora  Is a corresponding author
  1. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, France
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Cite this article as: eLife 2014;3:e02104 doi: 10.7554/eLife.02104

Abstract

The histone acetyltransferase (HAT) Mof is essential for mouse embryonic stem cells (mESC) pluripotency and early development. Mof is the enzymatic subunit of two different HAT complexes, MSL and NSL. The individual contribution of MSL and NSL to transcription regulation in mESCs is not well understood. Our genome-wide analysis show that i) MSL and NSL bind to specific and common sets of expressed genes, ii) NSL binds exclusively at promoters, iii) while MSL binds in gene bodies. Nsl1 regulates proliferation and cellular homeostasis of mESCs. MSL is the main HAT acetylating H4K16 in mESCs, is enriched at many mESC-specific and bivalent genes. MSL is important to keep a subset of bivalent genes silent in mESCs, while developmental genes require MSL for expression during differentiation. Thus, NSL and MSL HAT complexes differentially regulate specific sets of expressed genes in mESCs and during differentiation.

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Author details

  1. Sarina Ravens

    Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  2. Marjorie Fournier

    Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  3. Tao Ye

    Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  4. Matthieu Stierle

    Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  5. Doulaye Dembele

    Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  6. Virginie Chavant

    Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, Illkirch, France
    Competing interests
    The authors declare that no competing interests exist.
  7. Làszlò Tora

    Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104 - Inserm U 964, Université de Strasbourg, Illkirch, France
    For correspondence
    laszlo@igbmc.fr
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Danny Reinberg, Howard Hughes Medical Institute, New York University School of Medicine, United States

Publication history

  1. Received: December 17, 2013
  2. Accepted: June 3, 2014
  3. Accepted Manuscript published: June 4, 2014 (version 1)
  4. Version of Record published: June 17, 2014 (version 2)

Copyright

© 2014, Ravens et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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