Structure of cellular ESCRT-III spirals and their relationship to HIV budding
- Washington University School of Medicine, United States
Abstract
The ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission for trafficking into multivesicular bodies in the endocytic pathway and for the topologically related processes of viral budding and cytokinesis, but how they accomplish this remains unclear. Using deep-etch electron microscopy, we find that endogenous ESCRT-III filaments stabilized by depleting cells of Vps4 create uniform membrane-deforming conical spirals which are assemblies of specific ESCRT-III heteropolymers. To explore functional roles for ESCRT-III filaments, we examine HIV-1 Gag-mediated budding of virus-like particles and find that depleting Vps4 traps ESCRT-III filaments around nascent Gag assemblies. Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral.
Article and author information
Author details
Reviewing Editor
- Wesley Sundquist, University of Utah, United States
Version history
- Received: December 30, 2013
- Accepted: May 27, 2014
- Accepted Manuscript published: May 30, 2014 (version 1)
- Version of Record published: July 1, 2014 (version 2)
Copyright
© 2014, Cashikar et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 4,864
- views
-
- 534
- downloads
-
- 96
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Structure of cellular ESCRT-III spirals and their relationship to HIV budding
eLife 3:e02184.
https://doi.org/10.7554/eLife.02184
