(A) Schematic representation of MeCP2-EGFP fusion protein, showing the methyl-CpG binding domain (MBD, blue), transcriptional repression domain (TRD, red), a cluster of basic amino acids (yellow), …
(A) Using a Kaplan–Meier survival curve, both WT (black, n = 11) and R111G Tg (blue, n = 10) have a normal lifespan, whereas R111G mice (green, n = 10) phenocopy the premature lethality of the null …
(A) MeCP2-R111G does not bind to methyl-CpGs in vivo. Immunofluorescence for endogenous MeCP2 (anti-MeCP2 antibody, red), the transgene (anti-GFP antibody, green), and heterochromatic foci (puncta …
(A) A Kaplan–Meier survival curve shows that WT (black, n = 28) and R306C Tg (blue, n = 22) mice have a normal lifespan, while R306C (green, n = 21) mice have a shortened lifespan with 50% lethality …
(A–E) Behavioral tests were performed at 5 weeks of age to examine the R306C phenotype. At this age, WT (black, n = 18) and R306C Tg (blue, n = 17) mice are indistinguishable. Like the null mice …
(A) MeCP2-R306C binds methyl-CpGs in vivo. Immunofluorescence for endogenous MeCP2 (anti-MeCP2 antibody, red), the transgene (anti-GFP antibody, green), and heterochromatic foci (puncta within the …
(A) An overview of the lifespans of RTT mouse models emphasizes the fact that the R306C missense mutation is more detrimental than an earlier truncation at G273X. Full-length MeCP2 (gray) with the …