Phasic activation of ventral tegmental neurons increases response and pattern similarity in prefrontal cortex neurons
- National Institutes of Health, United States
Figures
Figure 1
Ca2+ imaging setup and calcium transients in response to VTA stimulation with and without anesthesia.
(A) Retrograde tracing. Fluorogold was injected into the prefrontal cortex (PFC). Cells in the secondary visual cortex lateral area (V2L) were labeled. Scale bar, 250 μm. (B) Anterograde tracing. …
Figure 2 with 1 supplement
Ca2+ transients in response to ventral tegmental area (VTA) stimulation.
(A) The left panel shows the population average of Ca2+ transients across the cells of a single animal. The right panel shows the Ca2+ transients of each single neuron using a color map according to …
Figure 2—figure supplement 1
Ca2+ transients in response to secondary visual cortex lateral area (V2L) stimulation.
(A) The left panel shows the population average of Ca2+ transients across the cells of a single animal. The right panel shows the Ca2+ transients of each single neuron indicated in a color map …
Figure 3 with 1 supplement
Long-lasting Ca2+ transients depend on D1 receptors.
(A and B) The effect of the D1 antagonist SCH23390 (1 mg/kg) (upper panel), the D2 antagonist eticlopride (0.5 mg/kg) (middle panel), and H2O, used as a control (bottom panel), on the long-lasting …
Figure 3—figure supplement 1
Short Ca2+ transients do not depend on D1 or D2 receptors but on glutamate receptors.
(A and B) The effect of D1 antagonist SCH23390 (1 mg/kg, upper panel), D2 antagonist eticlopride (0.5 mg/kg, middle panel) and H2O, used as a control (bottom panel), on the short Ca2+ transients …
Figure 4 with 2 supplements
Combined repetitive stimulation of the secondary visual cortex lateral area (V2L) and the ventral tegmental area (VTA) causes a modification of prefrontal cortex (PFC) neuronal response.
(A) Experimental design for stimulation of the V2L and VTA composed of three phases: pre-conditioning, conditioning, and test. Five pulses at 5 Hz and 10–15 pulses at 50 Hz were used for V2L and VTA …
Figure 4—figure supplement 1
Percentage distributions of neurons by dF/F in each conditioning group and reliability of calcium transients occurrence following three repetitive secondary visual cortex lateral area (V2L) stimulations.
(A) Percentage distributions of neurons, pooled from eight animals in each conditioning group, are shown. The number of neurons showing a dF/F value above 0.2 increased 2 hr after stimulation (right …
Figure 4—figure supplement 2
No correlation between Ca2+ influx evoked by ventral tegmental area (VTA) stimulation and neuronal activity increase in response to secondary visual cortex lateral area (V2L) stimulation.
Scatter plots of the Ca2+ transients evoked by VTA phasic stimulation from the 30 to 50 s time points against the increased value of dF/F, calculated by subtracting the dF/F value of ‘Before’ from …
Additional files
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Supplementary file 1
ImageJ macros for detecting individual cells.
- https://doi.org/10.7554/eLife.02726.011
