(A) Liver/body weight ratios of 1- to 3-month-old YapKIAlb-Cre mice (KI) and littermate controls (WT), n ≥ 11. ****p ≤ 0.0001. (B) Total hepatocyte numbers were determined by quantitative flow cytometry of primary hepatocytes isolated from 1- to 3-month-old mice of the indicated genotypes, n ≥ 14. (C) A representative image of H&E staining performed on liver sections of 6-week-old control (WT) and YapKIAlb-Cre mice (KI). (D) Primary hepatocytes were isolated by collagenase perfusion from YapKIAlb-Cre mice of indicated age groups, and percentage of GFPhigh hepatocytes was determined by flow cytometry; n ≥ 11. (E) Mice were injected with BrdU for 3 consecutive days and liver sections were stained with BrdU and GFP antibodies. Percent of BrdU+ hepatocytes was quantified in liver sections of the controls (WT) and within Yaplow (GFP−) and Yaphigh (GFP+) populations of the YapKIAlb-Cre livers. n ≥ 8. (F) TUNEL-positive nuclei were quantified on liver sections of control (WT) and YapKIAlb-Cre (KI) mice. 3 mice were used for each group and 4 images were taken for each mouse. Each dot represents cell count from each image. **p ≤ 0.01.