Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals
- University Medical Center, Georg-August-University Göttingen, Germany
- Institute for Microbiology and Genetics, Georg-August-University Göttingen, Germany
Abstract
Translational readthrough gives rise to low abundance proteins with C-terminal extensions beyond the stop codon. To identify functional translational readthrough, we estimated the readthrough propensity (RTP) of all stop codon contexts of the human genome by a new regression model in silico, identified a nucleotide consensus motif for high RTP by using this model, and analyzed all readthrough extensions in silico with a new predictor for peroxisomal targeting signal type 1 (PTS1). Lactate dehydrogenase B (LDHB) showed the highest combined RTP and PTS1 probability. Experimentally we show that at least 1.6% of the total cellular LDHB getting targeted to the peroxisome by a conserved hidden PTS1. The readthrough-extended lactate dehydrogenase subunit LDHBx can also co-import LDHA, the other LDH subunit into peroxisomes. Peroxisomal LDH is conserved in mammals and likely contributes to redox equivalent regeneration in peroxisomes.
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© 2014, Schueren et al.
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Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals
eLife 3:e03640.
https://doi.org/10.7554/eLife.03640
