1. Chromosomes and Gene Expression
  2. Plant Biology
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Transposition-mediated DNA re-replication in maize

  1. Jianbo Zhang
  2. Tao Zuo
  3. Dafang Wang
  4. Thomas Peterson  Is a corresponding author
  1. Iowa State University, United States
Research Article
  • Cited 9
  • Views 1,805
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Cite this article as: eLife 2014;3:e03724 doi: 10.7554/eLife.03724

Abstract

Every DNA segment in a eukaryotic genome normally replicates once and only once per cell cycle to maintain genome stability. We show here that this restriction can be bypassed through alternative transposition, a transposition reaction that utilizes the termini of two separate, nearby transposable elements (TEs). Our results suggest that alternative transposition during S phase can induce re-replication of the TEs and their flanking sequences. The DNA re-replication can spontaneously abort to generate double-strand breaks, which can be repaired to generate Composite Insertions composed of transposon termini flanking segmental duplications of various lengths. These results show how alternative transposition coupled with DNA replication and repair can significantly alter genome structure and may have contributed to rapid genome evolution in maize and possibly other eukaryotes.

Article and author information

Author details

  1. Jianbo Zhang

    Iowa State University, Ames, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Tao Zuo

    Iowa State University, Ames, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Dafang Wang

    Iowa State University, Ames, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Thomas Peterson

    Iowa State University, Ames, United States
    For correspondence
    thomasp@iastate.edu
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Bin Han, National Center for Gene Research, China

Publication history

  1. Received: June 18, 2014
  2. Accepted: November 17, 2014
  3. Accepted Manuscript published: November 18, 2014 (version 1)
  4. Version of Record published: December 16, 2014 (version 2)

Copyright

© 2014, Zhang et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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