1. Chromosomes and Gene Expression
  2. Genetics and Genomics
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Heterochromatin assembly and transcriptome repression by Set1 in coordination with a class II histone deacetylase

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Cite this article as: eLife 2014;3:e04506 doi: 10.7554/eLife.04506

Abstract

Histone modifiers play essential roles in controlling transcription and organizing eukaryotic genomes into functional domains. Here, we show that Set1, the catalytic subunit of the highly conserved Set1C/COMPASS complex responsible for histone H3K4 methylation (H3K4me), behaves as a repressor of the transcriptome largely independent of Set1C and H3K4me in the fission yeast Schizosaccharomyces pombe. Intriguingly, while Set1 is enriched at highly expressed and repressed loci, Set1 binding levels do not generally correlate with the levels of transcription. We show that Set1 is recruited by the ATF/CREB homolog Atf1 to heterochromatic loci and promoters of stress-response genes. Moreover, we demonstrate that Set1 coordinates with the class II histone deacetylase Clr3 in heterochromatin assembly at prominent chromosomal landmarks and repression of the transcriptome that includes Tf2 retrotransposons, noncoding RNAs, and regulators of development and stress-responses. Our study delineates a molecular framework for elucidating the functional links between transcriptome control and chromatin organization.

Article and author information

Author details

  1. David R Lorenz

    Department of Biology, Boston College, Chestnut Hill, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Lauren F Meyer

    Department of Biology, Boston College, Chestnut Hill, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Patrick J R Grady

    Department of Biology, Boston College, Chestnut Hill, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Michelle M Meyer

    Department of Biology, Boston College, Chestnut Hill, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Hugh P Cam

    Department of Biology, Boston College, Chestnut Hill, United States
    For correspondence
    hugh.cam@bc.edu
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Ali Shilatifard, Northwestern University Feinberg School of Medicine, United States

Publication history

  1. Received: August 26, 2014
  2. Accepted: December 12, 2014
  3. Accepted Manuscript published: December 15, 2014 (version 1)
  4. Version of Record published: January 14, 2015 (version 2)

Copyright

© 2014, Lorenz et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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