(A) A schematic drawing of the activation mechanism of the Ca2+ FRET biosensor. (B) Beads coated with Fn or BSA were seeded onto the cell and mechanical force was applied by pulling a Fn-coated bead …
Color images (upper panels) represent the YPet/ECFP emission ratio of the cytoplasmic Ca2+ biosensor. The color scale bars represent the range of emission ratio, with cold and hot colors indicating …
The time courses represent the YPet/ECFP emission ratio of cytoplasmic Ca2+ in HMSCs pretreated with (A) Thapsigargin (TG, 10 µM), a SERCA pump blocker, or (B) 2-APB (100 µM), an IP3R blocker. (C) …
The time courses represent the YPet/ECFP emission ratio of cytoplasmic Ca2+ in HMSCs in the absence of extracellular Ca2+ when these cells were pretreated with (A) 2 μM Cyto D (n = 8), (B) 1 μM Noc …
The time courses represent the YPet/ECFP emission ratio of cytoplasmic Ca2+ in HMSCs pretreated with (A) GdCl3 (5 µM), a broad spectrum Ca2+ channel inhibitor, (B) LaCl3 (100 µM), a broad spectrum …
(A) HMSCs expressing Ca2+ biosensor and transfected with non-targeting (NT) or TRPM7 siRNA were immunostained (upper images) or immunoblotted (lower panels) with polyclonal TRPM7 antibody to assess …
(A) The time course and (B) the color images of YPet/ECFP emission ratio in HMSCs expressing the D3ER biosensor before and after force application. The red arrows indicated episodes of Ca2+ release …
(A, C) Color images represent the YPet/ECFP emission ratio of the cytoplasmic Ca2+ biosensor in control cells treated by 2-APB only (n = 5) or those co-treated by Nifedipine (n = 5), Gd3+ (n = 3), …
(A) The inhibition of neither Src by PP1 (n = 4), FAK by PF228 (n = 4), nor PI3K by LY294002 (n = 3) abolished the force-induced cytosolic Ca2+ oscillations in HMSCs without extracellular Ca2+. (B) …