(A) Punctate distribution of GFP::RAB-3 in C. elegans motor neurons in wild-type animals (top). The GFP::RAB-3 puncta become diffuse in aex-3, rep-1, hmgs-1, and snt-1 mutants (lower panels). Yellow …
(A) Multiple snt-1 alleles show the diffuse GFP::RAB-3 phenotype. White arrows indicate the cell bodies. (B) GFP::RAB-3 expressed pan-neuronally under the control of the Prab-3 promoter displays …
(A) SNB-1::GFP puncta distribution in wild type and snt-1 mutants. (B) The synaptic enrichment of SNB-1::GFP puncta is indistinguishable in wild type and snt-1. Data are presented as mean ± SD; NS, …
(A) A GST-fused RBD domain of RIM2 binds active GTP-RAB-3. The amount of GTP-RAB-3 pulled down by RBD is decreased in both aex-3 and snt-1 animals. (B) Quantification of the GTP-RAB-3 level in wild …
(A) The punctate distribution of GFP::RAB-3 is restored in rbg-1;snt-1 animals, while the aex-3 phenotype could not be suppressed by mutation of rbg-1. Yellow arrows indicate the cell bodies along …
(A) Schematic representation of the rbg-1(ok1660) deletion mutation. (B) Schematic representation of the rbg-2(ok3195) deletion mutation. Solid boxes indicate exons and thin lines indicate introns. …
(A and B) mCherry::RBG-1 has a punctate distribution in wild type (A) but accumulates in cell bodies (yellow arrows) in unc-104 mutants (B). (C) mCherry::RBG-1 (red) is co-localized with SNB-1::GFP …
(A) RBG-1 is precipitated by the intracellular domain (C2AB) of SNT-1. (B) The SNT-1 intracellular domain is precipitated by RBG-1. (C) The C2B domain of SNT-1 binds to RBG-1. (D) SNT-1 without the …
(A) Ca2+ treatment diminishes the binding between RBG-1 and the intracellular domain (C2AB) of SNT-1. (B) Quantification of the relative binding between RBG-1 and the C2AB domain upon Ca2+ …
(A) The failure of RAB-3/SV dissociation caused by exocytosis mutants, including unc-2 and unc-13, is bypassed by mutation of snt-1. Yellow arrows indicate the cell bodies along the ventral cord. …