(A) Distance traveled as a function of time for a single myosin 5 molecule biotinylated at the N-terminus and labeled with a 20 nm streptavidin-functionalized gold particle. The lateral localisation …
(A) Sample trajectories at [ATP] = 10 µM and at 1000 frames/s. Arrow indicates direction of motion. Scale bar: 50 nm. (B) Comparison of myosin 5 activity recorded in this work with previous single …
(A) Representative distance time series of a myosin 5 molecule labelled with an atto-647N/streptavidin conjugate at the N-terminus and tracked using single molecule total internal fluorescence …
(A) Representative time trace segment of an event in which the leading head detaches from the actin filament and exhibits the intermediate state behaviour reported in the main text. (B) …
(A) Distance trace (upper panel) with the simultaneously recorded iSCAT contrast (lower panel). The red subset of the traces corresponds to the unbound head state. Inset: schematic illustrating how …
(A) 2D-trajectory of myosin 5 stepping along actin recorded simultaneously with 445 nm (blue trace) and 635 nm (red trace) illumination. Although the latter channel exhibits higher noise due to a …
One head was labeled with a 20 nm streptavidin-functionalized gold particle (red) and the other with a fluorescent quantum dot (blue). Reported values correspond to the standard deviation σ in the …
One head was labelled with a 20 nm streptavidin-functionalized gold particle (red) and the other with a fluorescent quantum dot (blue). Reported values correspond to the standard deviation σ in the …
(A) Sample 2D trajectories for a 20 nm gold particle immobilised on the surface and attached to the N-terminus of myosin 5a. The arrow indicates the direction of myosin movement. The trace shown is …
Data was collected at 100 Hz and 10 μM ATP, using 20 nm diameter gold nanoparticles. Number of steps analysed: 554. (B) The positional fluctuations of the A and B states presented as histograms of …
(A) Tracking of the scattering signal from the gold nanoparticle attached to the N-terminus (black) and fluorescence signal from the GFP moiety located at the C-terminus of the same myosin 5a …
(A) Histograms for the distances between A and B states for 20, 30 and 40 nm gold nanoparticle labels located at the N-terminus. Total number of steps recorded: 124, 103 and 116, respectively. (B) …
(A and B) Simultaneous observation of the AB transition and the transient state for right and left handed walking molecules. (C and D) Position of the transient state for the same molecule before …
Upper panel represents the transient state of the unbound head. Contour map of a two-dimensional histogram with a 10 × 10 nm2 bin width obtained from the 1000 frames/s data (N = 486). Lower panel …
(A) Contour maps of the transient state at three different ATP concentrations. Obtaining traces at high ATP concentration was challenging due to the small available field of view and rapid …
In all cases, data and fits for the pre-power stroke state are shown in red; data and fits for the post-power stroke state are shown in blue. At saturating (1 mM) ATP concentration (A) both dwell …
If the head searched the full spherical space, the average position would be simply about half way between the leading head and the next binding site. For a parallel orientation this would be 56 nm …
Contour map of a two-dimensional histogram with a 18 × 18 nm2 bin width was obtained using all, left- and right- handed walking traces, without flipping (left panel). The resulting contour plot …
(A) ATP binding to the trailing head (yellow) and its detachment releases strain stored in the molecule. (B) The bound head (black with a gold nanoparticle attached) performs its power stroke which …
The left panel presents the raw video format after referencing with the flat field image. The flat field accounts for illumination inhomogeneities and was generated as described in the Materials and …
The video was taken at 100 frames/s and at a magnification of 31.8 nm/pixel. The corresponding field of view shown is 2 × 2 μm2 and corresponds to a total recording time of 5 s. The video playback …