(A) Representative EPSP traces following stimulation of a single action potential in wild type and vGlut-OE animals; representative mEPSP traces in inset. (B) Representative EPSC traces following stimulation of a single action potential in wild type and vGlut-OE animals; representative mEPSC traces in inset. (C) vGlut-OE animals have increased mEPSP amplitudes (p < 0.0001) with no change in evoked EPSP amplitudes (p = 0.08), resulting in a decrease in calculated quantal content (p < 0.0001, n > 12 NMJ per genotype). (D) vGlut overexpression results in increased mEPSC amplitudes (p < 0.0001), but no significant change in evoked EPSC amplitudes, resulting in a decrease in calculated quantal content (p < 0.01, n > 8 per genotype, error bars = SEM). (E) The cumulative frequency of mEPSPs is shifted to greater amplitudes in vGlut-OE animals. (F) Quantal content is able to scale across an order of magnitude in response to perturbations that increase (vGlut2 overexpression) or decrease (PhTx treatment) quantal amplitude. An exponential function was fit to these data points with R2 = 0.7491. [Ca]e = 3 mM for (B) and (D), 0.3 mM for all others. Error bars = SEM for all.