Homeostatic synaptic depression is achieved through a regulated decrease in presynaptic calcium channel abundance
- University of California, San Francisco, United States
Figures
Figure 1
vGlut-OE animals display increased miniature EPSP amplitude and a compensatory decrease in vesicle release.
(A) Representative EPSP traces following stimulation of a single action potential in wild type and vGlut-OE animals; representative mEPSP traces in inset. (B) Representative EPSC traces following …
Figure 2
Homeostatic potentiation can be induced at NMJ already expressing presynaptic homeostatic depression (PHD).
(A) Representative evoked EPSP traces from wild type and vGlut-OE NMJ without and with the application of PhTx. Corresponding mEPSP traces in inset for each condition. (B) The addition of PhTx to …
Figure 3
Presynaptic homeostatic potentiation and depression operate through distinct molecular mechanisms.
(A) Representative single action potential stimulated EPSP traces from wild type and vGlut-OE animals in the presence of the drug Benzamil with corresponding mEPSP traces in the inset. (B) vGlut-OE …
Figure 4
Action potential waveforms do not change during presynaptic homeostasis.
(A) Normalized average AP traces comparing PhTx-treated, GluRIIA mutant, and vGlut-OE animals to WT animals. (B) AP width does not change in animals in which presynaptic homeostasis has been induced …
Figure 5
Effects of PHD on RRP size and Pr.
(A) Top: representative EPSC trains from wild type (left) and vGlut-OE (right) animals in response to 60 Hz stimulation (30 stimuli). Bottom: cumulative EPSC amplitude for the traces shown. The line …
Figure 6
Animals expressing PHD have reduced presynaptic calcium influx.
(A) Representative line-scans of WT and vGlut-OE boutons. Red arrowhead: moment of stimulation. (B) Example Ca2+ transients from WT and vGlut-OE animals. vGlut-OE transients are identical to WT when …
Figure 7
Animals expressing PHD have reduced CaV2.1 channel levels.
(A) Example EPSP traces of vGlut-OE animals with (right) and without (left) a Cac-GFP transgene; example mEPSPs in inset. (B) vGlut-OE animals expressing a Cac-GFP transgene had unchanged synaptic …
