The 5-Pase domain hydrolyzes phosphoinositides, while Sac1 plays an essential but non-catalytic role at synapses. Evoked EPSCs from wt, unc-26(s1710) mutant, and indicated transgenic strains were compared. Transgenes were GFP-tagged UNC-26 variants, including Sac1 dead (C378S,D380N; full-length UNC-26), 5-Pase dead (D716A, full-length UNC-26), and ∆Sac1 (residues 494–1113). Transgenes were driven by Prab-3. Representative traces (A) and summary data (B) for evoked EPSC amplitudes are shown for the indicated genotypes. ***, p < 0.0001 when compared to wt controls. ###, p < 0.0001 when compared to unc-26 mutants. The number of worms analyzed for each genotype is indicated in the bar graphs. Error bars indicate SEM.