Z projections of living (A–E) and fixed (F–J) C. elegans meiotic embryos viewed down the pole-to-pole spindle axis at metaphase I (A, B, F, G) or metaphase II (C, D, E, H, I, J). mCherry::Histone H2B and GFP::tubulin label the chromosomes and spindle, respectively, in live embryos. Fixed embryos were stained with DAPI, anti-tubulin antibody, and a LacO FISH probe that recognizes a LacO array integrated on the X chromosome. Asterisks indicate polar bodies. Insets show polar bodies that did not fit in the image frame. In (E), ‘s’ denotes a sperm outside of the embryo. Percentages are shown for each outcome (K, L). Bar = 5 μm.