(A) rsc2 Ts− alleles are suppressed by isw1Δ. An ISW1+ strain (YBC1231; rsc1Δ rsc2Δ [RSC1.URA3]) and an isw1Δ strain (YBC1479; rsc1Δ rsc2Δ isw1Δ [RSC1.URA]) were transformed with TRP1-marked RSC2 (p604), rsc2-V457M (p776), or rsc2-D461G (p777), streaked to SC-TRP + 5FOA to force loss of the RSC1 plasmid, and then spotted as 10-fold dilutions to YPD at 30°C, 33°C, and YPD containing 1.5% formamide (Form) or 12 μg/ml benomyl. (B) isw1Δ suppresses 6-azauracil (6AU) and MPA phenotypes of rsc2 mutations. YBC1231 (ISW1+) and YBC1479 (isw1Δ) were transformed with TRP1-marked RSC2 (p776), rsc2-V457M (p776), or rsc2-(YBC777), streaked to SC-TRP + 5FOA to force loss of the RSC1 plasmid, and then transformed with URA3-marked vector. Strains were then streaked to SC-URA medium containing 20 μg/ml MPA or 150 μg/ml 6AU. (C) isw1Δ suppresses additional RSC mutations but does not suppress snf2. WT (YBC62), isw1Δ (YBC1416), rsc2-V457M (YBC1111), rsc2-V457M isw1Δ (YBC2810), rsc33 (YBC906), rsc3-3 isw1Δ (YBC1485 p817), rsc4-2 (YBC1278), rsc4-2 isw1Δ (YBC2867), rsc7Δ (YBC1333), rsc7Δ isw1Δ (YBC2233), snf2Δ (YBC26), and snf2Δ isw1Δ (YBC2812) were spotted as 10-fold serial dilutions to YPD 30°C, 33°C, 35°C, 38°C, YPD containing 150 mM Hydroxyurea (HU), and YPGal + Antimycin A (AA). Figure 2—figure supplement 1 shows suppression of rsc2 alleles by catalytic isw1 and isw2Δ mutants.