(A) Notch RNAi driven in all hemocytes reduces the number of melanized sessile crystal cells observed upon heat shock treatment to the whole larva. A similar level of reduction is seen with SerrateRNAi but not with DeltaRNAi (n = 20). (B) Still images of a 3-hr video showing the induction of lozenge reporter expression in Notch activated hemocytes (arrows). (C) NotchRNAi reduces the proportion of Lz+ cells in sessile hemocytes quantified with P1 immunofluorescence (IF) staining (n = 11 samples). (D) Serrate RNAi driven only in Lz+ cells does not reduce the number of melanized sessile crystal cells seen upon heat shock treatment contrarily to two other drivers expressed in plasmatocytes, Eater-GAL4 and Pxn-GAL4 (n = 20 samples). In all graphics only female larvae are shown, error bars represent SEM, n.s. = non significant p-value, **p < 0.01, ***p < 0.001.