1. Developmental Biology
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Drosophila sessile hemocyte clusters are true hematopoietic tissues that regulate larval blood cell differentiation

  1. Alexandre B Leitão
  2. Élio Sucena  Is a corresponding author
  1. Instituto Gulbenkian de Ciência, Portugal
Research Article
  • Cited 47
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Cite this article as: eLife 2015;4:e06166 doi: 10.7554/eLife.06166

Abstract

Virtually all species of coelomate animals contain blood cells that display a division of labor necessary for homeostasis. This functional partition depends upon the balance between proliferation and differentiation mostly accomplished in the hematopoietic organs. In Drosophila melanogaster, the lymph gland produces plasmatocytes and crystal cells that are not released until pupariation. Yet, throughout larval development, both hemocyte types increase in numbers. Mature plasmatocytes can proliferate but it is not known if crystal cell numbers increase by self-renewal or by de novo differentiation. We show that new crystal cells in third instar larvae originate through a Notch-dependent process of plasmatocyte transdifferentiation. This process occurs in the sessile clusters and is contingent upon the integrity of these structures. The existence of this hematopoietic tissue, relying on structure-dependent signaling events to promote blood homeostasis, creates a new paradigm for addressing outstanding questions in Drosophila hematopoiesis and establishing further parallels with vertebrate systems.

Article and author information

Author details

  1. Alexandre B Leitão

    Instituto Gulbenkian de Ciência, Oeiras, Portugal
    Competing interests
    The authors declare that no competing interests exist.
  2. Élio Sucena

    Instituto Gulbenkian de Ciência, Oeiras, Portugal
    For correspondence
    esucena@igc.gulbenkian.pt
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Utpal Banerjee, University of California, Los Angeles, United States

Publication history

  1. Received: December 19, 2014
  2. Accepted: February 3, 2015
  3. Accepted Manuscript published: February 4, 2015 (version 1)
  4. Version of Record published: March 12, 2015 (version 2)

Copyright

© 2015, Leitão & Sucena

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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