Reverse-correlation analysis of navigation dynamics in Drosophila larva using optogenetics
- Harvard University, United States
- Yale University, United States
Figures
Figure 1 with 1 supplement
Experimental method for reverse-correlation analysis using optogenetics.
(A) Larvae navigate by alternating between two basic motor states: runs and turns. The navigation strategy of the animal can be characterized by finding the mathematical functions, fr → t and ft → r …
Figure 1—figure supplement 1
Optogenetic activation of OK6-Gal4 motor neurons.
To test if our experimental setup robustly activates CsChrimson, we expressed it in motor neurons using the OK6-Gal4 driver (Sanyal, 2009). Effective activation would result in most muscles of the …
Figure 2
ORNs evoked navigation strategy.
(A) Event-triggered stimulus histories for run-to-turn (left panel) and turn-to-run (right panel) transitions exhibited by Or42a>CsChrimson larvae subjected to random optogenetic stimulation as …
Figure 3 with 2 supplements
Linear-nonlinear (LN) models of behavior.
(A) Estimating the static nonlinear function for run-to-turn and turn-to-run transitions exhibited by Or45a>CsChrimson larvae. Linear prediction using the event-triggered stimulus histories from Figu…
Figure 3—figure supplement 1
LN models of Gr21a and Gr10a.
(A) Estimating the static nonlinear function for run-to-turn and turn-to-run transitions exhibited by Gr21a>CsChrimson larvae (left panels). Linear prediction using the event-triggered stimulus …
Figure 3—figure supplement 2
LN models of Or42a and Gr2a.
(A) Estimating the static nonlinear function for run-to-turn and turn-to-run transitions exhibited by Or42a>CsChrimson larvae (left panels). Linear prediction using the event-triggered stimulus …
Figure 4 with 1 supplement
Distinct navigation dynamics.
(A) Event-triggered stimulus histories exhibited by Gr21a>CsChrimson larvae. Linear filters of Gr21a neurons. Consistent with a repulsive response, the likelihood of optogenetic activation increases …
Figure 4—figure supplement 1
Statistical analysis of behavioral dynamics.
Each dot shown in Figure 4B, D is a probability but is also the mean of the distribution of larvae undergoing a run-to-turn transition if we consider each larvae undergoing this transition as 1 and …
Figure 5 with 2 supplements
Reverse-correlation analysis of bitter-sensing gustatory receptor neurons (GRNs).
Event-triggered stimulus histories exhibited by GrX>CsChrimson larvae using a set of GAL4 drivers that express in different subsets of GRNs. The cellular identities describing each expression …
Figure 5—figure supplement 1
Statistical analysis of Gr9a and Gr94a triggered average.
The triggered averages of Gr9a and Gr94a showed very weak response. Because of this, we tested whether their observed behavior is significantly different than the control. Each point of the …
Figure 5—figure supplement 2
Normalized speed of Gr lines.
The normalized speed of each Gr line is shown. Normalized speed is computed using standard score. In all cases, the slowdown initiation happens 1 s prior to the transition (t-test p < 0.01). Red …
