Changes in FLC (A), VIN3 (B) and (C) FT expression were determined directly after 6 weeks of cold exposure (T0) and again after 10 (T10) and 30 (T30) days subsequent growth at 20°C. Two-tailed Student's t-test results: *p < 0.05, ***p < 0.005. n = 3. Error bars = ±S.D. (D) H3K27me3 levels over the FLC locus were higher for Lov-1 after 6 weeks vernalization at 8°C than 14°C or 5°C (samples were harvested 30 days post cold). **** p < 0.0001, Wilcoxon matched-pairs signed rank test on measurements for 12 primer pairs. Error bars = ±S.E.M. NV = nonvernalized, DNF = did not flower and ns = not significant.