The circulatory system enables blood to move around the body and deliver substances including nutrients and oxygen to the cells that need them. In the embryos of animals with a backbone, blood flows from the heart through the aorta into branching smaller vessels to the cells. The blood then gets collected by progressively bigger vessels and flows back to the heart via the cardinal vein. The cells that make up these blood vessels develop from cells called angioblasts—but first, during development these angioblasts must move to the place where the vessels will form.

A protein called Vascular endothelial growth factor (VEGF) had been suggested to help guide and align the angioblasts as the embryo develops. Now, Helker, Schuermann et al. have examined developing zebrafish embryos using new technologies. This revealed that VEGF is in fact not essential for the dorsal aorta and cardinal vein to develop. Instead, the angioblasts only move to the correct part of the embryo if they can produce the Apelin receptor protein, which forms part of a signaling pathway.

There are two hormones—called Apelin and Elabela—that can bind to and activate the Apelin receptor. Helker, Schuermann et al. show that Elabela alone is needed to guide the angioblasts to the right part of the embryo during blood vessel development. However, in embryos where there is not enough Elabela, the Apelin hormone can compensate for this deficiency and the first blood vessels will later develop correctly. Future research will address whether this signaling pathway not only guides angioblasts to establish a circulatory system, but also guides blood vessel growth. As blood vessel growth is very relevant to human disease, identifying the mechanisms that regulate it will have an impact on biomedical research.

In the vertebrate embryo, the formation of the large axial vessels, namely the dorsal aorta (DA) and the cardinal vein (CV), establishes a first circulatory loop and thereby the core of the developing cardiovascular system. Angioblasts are initially specified in the lateral plate mesoderm and migrate between the somites towards the midline, where they coalesce and assemble the DA and the CV underneath the notochord (NC) (Figure 1A,B–F, Video 1).

Figure 1 with 2 supplements see all

Download asset Open asset

Figure 1—source movie 1

Time-lapse movie showing angioblast (green) migration to the midline analyzed using Tg(fli1a:EGFP)^y1 embryos injected with H2B-mCherry mRNA (purple, all nuclei).

https://doi.org/10.7554/eLife.06726.004

Download elife-06726-fig1-data1-v2.avi

Video 1

Download asset

It has been previously proposed that this process is regulated by Vascular endothelial growth factor A (VEGF-A) (Coultas et al., 2005; Verma et al., 2010; Gore et al., 2012), a master regulator of vascular growth in the embryonic and adult organism. In Xenopus embryos, transcripts for the vegfa gene are expressed in the midline and migrating angioblasts were guided towards sites of ectopic Vegfa expression (Cleaver and Krieg, 1998). Global inactivation of the genes encoding VEGF-A or the corresponding receptor VEGFR2 in mice led to strong vascular defects affecting the whole vascular network including the DA (Shalaby et al., 1995; Ferrara et al., 1996). Based on these data it was concluded that in vertebrates VEGF-A signaling regulates angioblast migration to the midline (Coultas et al., 2005; Verma et al., 2010; Gore et al., 2012). To get direct, mechanistic and dynamic insight into angioblast migration and formation of the great vessels, we decided to investigate these processes in zebrafish embryos.

As previously published (Nicoli et al., 2008), expression analysis of vegfaa, the gene for the main VEGF-A ortholog in zebrafish, showed the presence of transcripts in the somites between 12 and 15 hr post fertilization (hpf), that is the stage when angioblasts migrate to the midline (see Figure 1B–F). To analyze whether Vegfa signaling, as previously proposed, is required for angioblast migration to the midline, we analyzed loss-of-function zebrafish embryos with deficiencies in the Vegfa signaling pathway resulting from genomic mutations as well as morpholino (MO) -mediated knockdown. To rule out developmental delays, we counted the somites of the embryos and fixed them for confocal analysis, when they had developed 16–17 somites (equivalent to 17 hpf). Surprisingly, neither Vegfa ligand depletion (vegfaa/vegfab MO) nor a null mutation in the gene encoding the VEGFR2 ortholog (kdrl) interfered with angioblast migration in zebrafish embryos (Figure 1H,J), although Vegfa signaling dependent phenotypes, like failure to form the intersegmental vessels, could be observed after 24 hpf (Figure 1—figure supplement 1). Additionally, pharmacological inhibition of Vegfr2 signaling from 6 to 17 hpf did not impair angioblast migration to the midline (Figure 1—figure supplement 1). As activity of Sonic Hedgehog (Shh) in the midline has been shown to induce vegfaa expression (Lawson et al., 2002), we repeated the published experiments and blocked this process by chemical inhibition of Shh signaling using cyclopamine. In line with our previous experiments, angioblast migration was not affected after Shh inhibition (Figure 1—figure supplement 2). The sum of these data strongly indicates that Vegfa signaling is dispensable for angioblast specification or migration in zebrafish.

In order to identify the endogenous signal(s) guiding angioblast migration, we further analyzed the expression pattern of factors involved in cell motility and directed cell migration. As previously published (Zeng et al., 2007), we detected the expression of Apelin, a short secreted peptide encoded by the apln gene, by the NC (Figure 2—figure supplement 1C). At the same time, transcripts of its two paralogous receptors apelin receptor a (aplnra) and apelin receptor b (aplnrb) were present in angioblasts (Figure 2—figure supplement 1A,B; [Scott et al., 2007; Zeng et al., 2007]). Using MO-mediated gene knockdown, we observed that both aplnra and aplnrb are important for midline migration of angioblasts. While absence of each individual receptor reduced the efficiency of migration towards the midline, double depletion completely abolished this process (Figure 2A, Video 2). We used TALEN or CRISPR/Cas9-mediated gene editing (Hwang et al., 2013; Jao et al., 2013) to introduce mutations in either the aplnra or the aplnrb gene (Figure 2—figure supplement 2). As our MO-based analysis already indicated an additive effect of both receptor genes, we analyzed the phenotypes of the offspring of aplnra^+/−;aplnrb^+/− double heterozygous parents. The embryos were all individually scored for their phenotype regarding angioblast midline migration into four categories (normal, mild, strong, stronger; Figure 2B) and then subjected to genotyping. Homozygous mutant embryos phenocopied the MO-induced phenotypes, additional loss of one or two copies of the second receptor increased the severity of the phenotype (Figure 2C). Additionally, we detected aplnrb transcripts together with the angioblast specification marker etv2 indicating that both genes are expressed very early during angioblast specification. However, when we analyzed aplnra/aplnrb double deficient embryos, no difference in etv2 expression was detectable by in situ hybridization indicating that Apln receptors only regulate angioblast migration, but not specification (Figure 2—figure supplement 1E).

Figure 2 with 2 supplements see all

Download asset Open asset

Figure 2—source data 1

Excel table showing the phenotype categories and the number of embryos for each genotype in these phenotypic categories (for the apln/ela double mutant analysis).

https://doi.org/10.7554/eLife.06726.009

Download elife-06726-fig2-data1-v2.xlsx

Video 2

Download asset

Next, we analyzed the requirement for the ligand Apln by MO-mediated gene knockdown and, unexpectedly, observed no difference to control MO injected embryos (Figure 2A). To obtain undisputable genetic confirmation of this result, we again used gene editing to introduce mutations in the apln gene (Figure 2—figure supplement 2, most likely resulting in functional null mutants). Consistent with the MO-mediated depletion, homozygous apln^mu267 mutant embryos (Figure 2A) showed no vasculogenesis defects, which indicated that endogenous Apln is not sufficient to regulate angioblast migration to the midline.

Previous studies have identified a requirement for Apln receptors in myocardial development in zebrafish (Scott et al., 2007; Zeng et al., 2007) and mice (Charo et al., 2009), which could not completely be phenocopied by Apln deficiency (Scott et al., 2007; Zeng et al., 2007; Charo et al., 2009). These results led to the proposal that Apln may not be the only ligand for Aplnrs. Recently, a novel peptide hormone named Elabela (Ela, also known as Apela or Toddler) was identified in zebrafish and shown to bind and activate Aplnrs (Chng et al., 2013; Pauli et al., 2014). By in situ hybridization we detected ela expression by the NC (Figure 2—figure supplement 1D, [Pauli et al., 2014]), consistent with a possible role in attracting angioblasts to the midline. We next analyzed angioblast migration in zebrafish embryos carrying a homozygous null mutation in the ela gene (ela^br13). Ela deficiency led to a strong impairment of angioblast migration (Figure 2A), indicating that indeed Ela-Aplnr represent a novel bona fide ligand–receptor pair and therefore a novel signaling pathway regulating angioblast migration.

Given that the ela^-/- phenotype was not as severe as the depletion of both receptors and that apln expression increases later in the NC (while ela expression becomes progressively reduced, Figure 2—figure supplement 1C,D), we analyzed the phenotypes of the offspring of apln^+/−;ela^+/− double heterozygous parents. The embryos were all individually scored for their phenotype regarding angioblast midline migration into four categories (normal, mild, strong, severe) and then subjected to genotyping. Interestingly a mild phenotype was observed, when either one ela or both apln alleles were mutant, a strong phenotype when ela was homozygously mutant with increasing severity (severe phenotype) upon loss of the apln alleles (Figure 2D,E). Homozygous ela^−/−; apln^−/− double mutants phenocopied apln receptor deficiency (compare in Figure 2A,B). The observed dose dependency is indicative, that in this context Ela and Apln might act as novel chemoattractants for angioblast migration to the midline, with Ela acting as the major endogenous attractant and Apln having a minor additive role.

Previous analysis of zebrafish embryos lacking the NC showed, that signals from the midline guide angioblast migration (Fouquet et al., 1997; Sumoy et al., 1997). We could show that indeed angioblast migration as well as ela expression is perturbed in the zebrafish mutants lacking a NC (notochord homeobox, noto; previous name: floating head) or exhibiting defective NC precursor differentiation (T, brachyury homolog a, ta; previous name: no tail) (Figure 3) (Halpern et al., 1993; Odenthal et al., 1996). While angioblasts in noto^tk241 mutant embryos failed to migrate to the midline, ta^b160 angioblasts migrated towards the midline but failed to reach this structure (Figure 3A). In line with these results, ela expression was strongly diminished in the ta^b160 midline and almost absent in noto^tk241 mutant embryos (Figure 3B). We next wanted to test whether midline ela expression would be sufficient to restore angioblast migration in ta^b160 mutants. For this purpose, plasmid DNA with a shh promoter fragment enabling ela expression in the floorplate was injected together with Tol2 transposase mRNA to obtain mosaic Ela overexpression in the midline. Indeed, Ela overexpression did restore angioblast migration to the midline in ta^b160 embryos (Figure 3C), demonstrating that midline Ela expression is not only necessary, but sufficient to regulate angioblast migration during vasculogenesis.

Figure 3

Download asset Open asset

Next, we wanted to analyze the function of Ela-Aplnr signaling more mechanistically. Recently, it was published that Ela does not act as a chemoattractant, but instead acts as a motogen in regulating mesoderm migration during gastrulation (Pauli et al., 2014). In contrast, the aplnr deficient (aplra/b MO injected) angioblasts were still partially motile and did send out filopodia, but failed to migrate to the midline (Video 2). To test whether Ela does act as a chemoattractant or just enables motility of angioblasts, we overexpressed Ela at ectopic locations in noto or ta mutant embryos, as both of these lack endogenous ela and apln expression in the NC. We used a heatshock promoter to control timing of expression and labeled Ela overexpressing cells by coexpression of RFP through an internal ribosome entry site (IRES) element (Figure 4). We measured the distance of control (Cherry) or Ela/RFP expressing cells from angioblasts in a 40 μm radius of the red labeled cell and considered an angioblast as attracted, if it was less than 5 μm away from it. While only 20% of the angioblast were found in the vicinity of control cells, more than 52% of the angioblasts were close to Ela overexpressing cells (Figure 4D,G). Together with the observed dose dependency of Ela-Apln, the motility of Aplnr deficient embryos and the rescue of NC mutants by midline Ela expression, our data support the interpretation, that Ela acts as a chemoattractant for angioblasts during midline migration.

Figure 4

Download asset Open asset

To prove that Ela-Aplnr signaling is directly required in angioblasts and rule out that the observed migration defects were caused by changes in other cell types, we performed transplantation experiments. Mini-ruby injected (red fluorescence) transgenic fli1a:EGFP positive (angioblasts, green) cells from control MO injected, aplnra/b MO injected or wild type (WT) donor embryos were transplanted into either WT or aplnr loss-of-function (apnlra/b MO injected) host embryos (Figure 5A). Analysis of angioblast migration to the midline at 17 hpf revealed that in average 93.6% of control MO-injected transplanted angioblasts migrated to the midline in a WT host embryo (Figure 5B,C; n = 39 GFP+ angioblasts, 7 embryos). In contrast, only 9.4% of aplnra/b MO injected angioblasts arrived at the midline, whereas the majority of these cells remained in more lateral positions (Figure 5B,D; n = 62 GFP+ angioblasts, 5 embryos). Transplanted WT angioblasts were perfectly capable to migrate to the midline in an aplnr deficient environment (average of 81.9%, Figure 5B,E; n = 15 GFP+ angioblasts, 6 embryos), which shows that there is indeed a cell autonomous requirement for Aplnr signaling in angioblasts.

Figure 5

Download asset Open asset

Figure 5—source data 1

Statistical analysis and single values for the number of angioblasts and embryos analyzed.

https://doi.org/10.7554/eLife.06726.016

Download elife-06726-fig5-data1-v2.xlsx

Based on the sum of the results presented above, we propose that Ela expression at the embryonic midline provides a chemoattractive signal for Aplnr-positive angioblasts. While Ela and Apln both signal to the Apln receptors, the initial ela expression at the midline appears stronger than apln, which peaked at later developmental time points (Figure 2—figure supplement 1C,D). Thus, ela and apln may have partially redundant functions, but are required at different stages of vascular morphogenesis. Surprisingly, while Ela-Aplnr signaling was strictly required for the guided migration of angioblasts, this process was not controlled by Vegfa-mediated signaling.

Our studies identified Ela-Aplnr signaling as a novel signaling pathway for angioblasts regulating vascular patterning in the developing vertebrate embryo. As therapeutic intervention with vascular growth is clinically highly relevant and, so far, predominantly focuses on the VEGF-A signaling cascade, the Ela-Aplnr signaling pathway may also represent a novel therapeutic target in human disease.

1. 1. Bussmann J
   2. Bos FL
   3. Urasaki A
   4. Kawakami K
   5. Duckers HJ
   6. Schulte-Merker S

   (2010) Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk

   Development 137:2653–2657.

   https://doi.org/10.1242/dev.048207

   • Google Scholar
2. 1. Cermak T
   2. Doyle EL
   3. Christian M
   4. Wang L
   5. Zhang Y
   6. Schmidt C
   7. Baller JA
   8. Somia NV
   9. Bogdanove AJ
   10. Voytas DF

   (2011) Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

   Nucleic Acids Research 39:e82.

   https://doi.org/10.1093/nar/gkr218

   • Google Scholar
3. 1. Charo DN
   2. Ho M
   3. Fajardo G
   4. Kawana M
   5. Kundu RK
   6. Sheikh AY
   7. Finsterbach TP
   8. Leeper NJ
   9. Ernst KV
   10. Chen MM
   11. Ho YD
   12. Chun HJ
   13. Bernstein D
   14. Ashley EA
   15. Quertermous T

   (2009) Endogenous regulation of cardiovascular function by apelin-APJ

   American Journal of Physiology. Heart and Circulatory Physiology 297:H1904–H1913.

   https://doi.org/10.1152/ajpheart.00686.2009

   • Google Scholar
4. 1. Chng SC
   2. Ho L
   3. Tian J
   4. Reversade B

   (2013) ELABELA: a hormone essential for heart development signals via the apelin receptor

   Developmental Cell 27:672–680.

   https://doi.org/10.1016/j.devcel.2013.11.002

   • Google Scholar
5. 1. Cleaver O
   2. Krieg PA

   (1998)

   VEGF mediates angioblast migration during development of the dorsal aorta in Xenopus

   Development 125:3905–3914.

   • Google Scholar
6. 1. Coultas L
   2. Chawengsaksophak K
   3. Rossant J

   (2005) Endothelial cells and VEGF in vascular development

   Nature 438:937–945.

   https://doi.org/10.1038/nature04479

   • Google Scholar
7. 1. Ferrara N
   2. Carver-Moore K
   3. Chen H
   4. Dowd M
   5. Lu L
   6. O'Shea KS
   7. Powell-Braxton L
   8. Hillan KJ
   9. Moore MW

   (1996) Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene

   Nature 380:439–442.

   https://doi.org/10.1038/380439a0

   • Google Scholar
8. 1. Fouquet B
   2. Weinstein BM
   3. Serluca FC
   4. Fishman MC

   (1997) Vessel patterning in the embryo of the zebrafish: guidance by notochord

   Developmental Biology 183:37–48.

   https://doi.org/10.1006/dbio.1996.8495

   • Google Scholar
9. 1. Gordon K
   2. Schulte D
   3. Brice G
   4. Simpson MA
   5. Roukens MG
   6. van Impel A
   7. Connell F
   8. Kalidas K
   9. Jeffery S
   10. Mortimer PS
   11. Mansour S
   12. Schulte-Merker S
   13. Ostergaard P

   (2013) Mutation in vascular endothelial growth factor-C, a ligand for vascular endothelial growth factor receptor-3, is associated with autosomal dominant milroy-like primary lymphedema

   Circulation Research 112:956–960.

   https://doi.org/10.1161/CIRCRESAHA.113.300350

   • Google Scholar
10. 1. Gore AV
    2. Monzo K
    3. Cha YR
    4. Pan W
    5. Weinstein BM

    (2012) Vascular development in the zebrafish

    Cold Spring Harbor Perspectives in Medicine 2:a006684.

    https://doi.org/10.1101/cshperspect.a006684

    • Google Scholar
11. 1. Halpern ME
    2. Ho RK
    3. Walker C
    4. Kimmel CB

    (1993) Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation

    Cell 75:99–111.

    https://doi.org/10.1016/S0092-8674(05)80087-X

    • Google Scholar
12. 1. Helker CS
    2. Schuermann A
    3. Karpanen T
    4. Zeuschner D
    5. Belting HG
    6. Affolter M
    7. Schulte-Merker S
    8. Herzog W

    (2013) The zebrafish common cardinal veins develop by a novel mechanism: lumen ensheathment

    Development 140:2776–2786.

    https://doi.org/10.1242/dev.091876

    • Google Scholar
13. 1. Hesselson D
    2. Anderson RM
    3. Beinat M
    4. Stainier DY

    (2009) Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling

    Proceedings of the National Academy of Sciences of USA 106:14896–14901.

    https://doi.org/10.1073/pnas.0906348106

    • Google Scholar
14. 1. Hogan BM
    2. Bos FL
    3. Bussmann J
    4. Witte M
    5. Chi NC
    6. Duckers HJ
    7. Schulte-Merker S

    (2009) Ccbe1 is required for embryonic lymphangiogenesis and venous sprouting

    Nature Genetics 41:396–398.

    https://doi.org/10.1038/ng.321

    • Google Scholar
15. 1. Hwang WY
    2. Fu YF
    3. Reyon D
    4. Maeder ML
    5. Tsai SQ
    6. Sander JD
    7. Peterson RT
    8. Yeh JRJ
    9. Joung JK

    (2013) Efficient genome editing in zebrafish using a CRISPR-Cas system

    Nature Biotechnology 31:227–229.

    https://doi.org/10.1038/nbt.2501

    • Google Scholar
16. 1. Jao LE
    2. Wente SR
    3. Chen W

    (2013) Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

    Proceedings of the National Academy of Sciences of USA 110:13904–13909.

    https://doi.org/10.1073/pnas.1308335110

    • Google Scholar
17. 1. Kawakami K
    2. Takeda H
    3. Kawakami N
    4. Kobayashi M
    5. Matsuda N
    6. Mishina M

    (2004) A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish

    Developmental Cell 7:133–144.

    https://doi.org/10.1016/j.devcel.2004.06.005

    • Google Scholar
18. 1. Kimmel CB
    2. Ballard WW
    3. Kimmel SR
    4. Ullmann B
    5. Schilling TF

    (1995) Stages of embryonic development of the zebrafish

    Developmental Dynamics 203:253–310.

    https://doi.org/10.1002/aja.1002030302

    • Google Scholar
19. 1. Larson JD
    2. Wadman SA
    3. Chen E
    4. Kerley L
    5. Clark KJ
    6. Eide M
    7. Lippert S
    8. Nasevicius A
    9. Ekker SC
    10. Hackett PB
    11. Essner JJ

    (2004) Expression of VE-cadherin in zebrafish embryos: a new tool to evaluate vascular development

    Developmental Dynamics 231:204–213.

    https://doi.org/10.1002/dvdy.20102

    • Google Scholar
20. 1. Lawson ND
    2. Vogel AM
    3. Weinstein BM

    (2002) sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation

    Developmental Cell 3:127–136.

    https://doi.org/10.1016/S1534-5807(02)00198-3

    • Google Scholar
21. 1. Lawson ND
    2. Weinstein BM

    (2002) In vivo imaging of embryonic vascular development using transgenic zebrafish

    Developmental Biology 248:307–318.

    https://doi.org/10.1006/dbio.2002.0711

    • Google Scholar
22. 1. Nasevicius A
    2. Ekker SC

    (2000) Effective targeted gene 'knockdown' in zebrafish

    Nature Genetics 26:216–220.

    https://doi.org/10.1038/79951

    • Google Scholar
23. 1. Nicoli S
    2. Tobia C
    3. Gualandi L
    4. De Sena G
    5. Presta M

    (2008) Calcitonin receptor-like receptor guides arterial differentiation in zebrafish

    Blood 111:4965–4972.

    https://doi.org/10.1182/blood-2007-10-118166

    • Google Scholar
24. 1. Ober EA
    2. Olofsson B
    3. Makinen T
    4. Jin SW
    5. Shoji W
    6. Koh GY
    7. Alitalo K
    8. Stainier DY

    (2004) Vegfc is required for vascular development and endoderm morphogenesis in zebrafish

    EMBO Rep 5:78–84.

    https://doi.org/10.1038/sj.embor.7400047

    • Google Scholar
25. 1. Odenthal J
    2. Haffter P
    3. Vogelsang E
    4. Brand M
    5. van Eeden FJ
    6. Furutani-Seiki M
    7. Granato M
    8. Hammerschmidt M
    9. Heisenberg CP
    10. Jiang YJ
    11. Kane DA
    12. Kelsh RN
    13. Mullins MC
    14. Warga RM
    15. Allende ML
    16. Weinberg ES
    17. Nüsslein-Volhard C

    (1996)

    Mutations affecting the formation of the notochord in the zebrafish, Danio rerio

    Development 123:103–115.

    • Google Scholar
26. 1. Pauli A
    2. Norris ML
    3. Valen E
    4. Chew GL
    5. Gagnon JA
    6. Zimmerman S
    7. Mitchell A
    8. Ma J
    9. Dubrulle J
    10. Reyon D
    11. Tsai SQ
    12. Joung JK
    13. Saghatelian A
    14. Schier AF

    (2014) Toddler: an embryonic signal that promotes cell movement via Apelin receptors

    Science 343:1248636.

    https://doi.org/10.1126/science.1248636

    • Google Scholar
27. 1. Santoro MM
    2. Samuel T
    3. Mitchell T
    4. Reed JC
    5. Stainier DY

    (2007) Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis

    Nature Genetics 39:1397–1402.

    https://doi.org/10.1038/ng.2007.8

    • Google Scholar
28. 1. Scott IC
    2. Masri B
    3. D'Amico LA
    4. Jin SW
    5. Jungblut B
    6. Wehman AM
    7. Baier H
    8. Audigier Y
    9. Stainier DY

    (2007) The g protein-coupled receptor agtrl1b regulates early development of myocardial progenitors

    Developmental Cell 12:403–413.

    https://doi.org/10.1016/j.devcel.2007.01.012

    • Google Scholar
29. 1. Shalaby F
    2. Rossant J
    3. Yamaguchi TP
    4. Gertsenstein M
    5. Wu XF
    6. Breitman ML
    7. Schuh AC

    (1995) Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice

    Nature 376:62–66.

    https://doi.org/10.1038/376062a0

    • Google Scholar
30. 1. Sumoy L
    2. Keasey JB
    3. Dittman TD
    4. Kimelman D

    (1997) A role for notochord in axial vascular development revealed by analysis of phenotype and the expression of VEGR-2 in zebrafish flh and ntl mutant embryos

    Mechanisms of Development 63:15–27.

    https://doi.org/10.1016/S0925-4773(97)00671-0

    • Google Scholar
31. 1. Verma A
    2. Bhattacharya R
    3. Remadevi I
    4. Li K
    5. Pramanik K
    6. Samant GV
    7. Horswill M
    8. Chun CZ
    9. Zhao B
    10. Wang E
    11. Miao RQ
    12. Mukhopadhyay D
    13. Ramchandran R
    14. Wilkinson GA

    (2010) Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity

    Blood 115:4614–4622.

    https://doi.org/10.1182/blood-2009-10-248856

    • Google Scholar
32. Book

    1. Westerfield M

    (1993)

    The Zebrafish Book

    Oxford University Press, Oxford, UK.

    • Google Scholar
33. 1. Zeng XX
    2. Wilm TP
    3. Sepich DS
    4. Solnica-Krezel L

    (2007) Apelin and its receptor control heart field formation during zebrafish gastrulation

    Developmental Cell 12:391–402.

    https://doi.org/10.1016/j.devcel.2007.01.011

    • Google Scholar
34. 1. Zhang S
    2. Cao Z
    3. Tian H
    4. Shen G
    5. Ma Y
    6. Xie H
    7. Liu Y
    8. Zhao C
    9. Deng S
    10. Yang Y
    11. Zheng R
    12. Li W
    13. Zhang N
    14. Liu S
    15. Wang W
    16. Dai L
    17. Shi S
    18. Cheng L
    19. Pan Y
    20. Feng S
    21. Zhao X
    22. Deng H
    23. Yang S
    24. Wei Y

    (2011) SKLB1002, a novel potent inhibitor of VEGF receptor 2 signaling, inhibits angiogenesis and tumor growth in vivo

    Clinical Cancer Research 17:4439–4450.

    https://doi.org/10.1158/1078-0432.CCR-10-3109

    • Google Scholar

Author details

1. Christian SM Helker

   University of Muenster, Muenster, Germany

   Contribution

   CSMH, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article

   Contributed equally with

   Annika Schuermann

   For correspondence

   Christian.Helker@mpi-bn.mpg.de

   Competing interests

   The authors declare that no competing interests exist.

2. Annika Schuermann

   University of Muenster, Muenster, Germany

   Contribution

   AS, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article

   Contributed equally with

   Christian SM Helker

   Competing interests

   The authors declare that no competing interests exist.

3. Cathrin Pollmann

   Max Planck Institute for Molecular Biomedicine, Muenster, Germany

   Contribution

   CP, Analysis and interpretation of data, Drafting or revising the article

   Competing interests

   The authors declare that no competing interests exist.

4. Serene C Chng

   Institute of Medical Biology, Human Genetics and Embryology Laboratory, A*STAR, Singapore, Singapore

   Contribution

   SCC, Contributed preliminary data, Drafting or revising the article, Contributed unpublished essential data or reagents

   Competing interests

   The authors declare that no competing interests exist.

5. Friedemann Kiefer

   1. Max Planck Institute for Molecular Biomedicine, Muenster, Germany
   2. Cells-in-Motion Cluster of Excellence, University of Muenster, Muenster, Germany

   Contribution

   FK, Analysis and interpretation of data, Drafting or revising the article

   Competing interests

   The authors declare that no competing interests exist.

6. Bruno Reversade

   Institute of Medical Biology, Human Genetics and Embryology Laboratory, A*STAR, Singapore, Singapore

   Contribution

   BR, Drafting or revising the article, Contributed unpublished essential data or reagents

   Competing interests

   The authors declare that no competing interests exist.

7. Wiebke Herzog

   1. University of Muenster, Muenster, Germany
   2. Max Planck Institute for Molecular Biomedicine, Muenster, Germany
   3. Cells-in-Motion Cluster of Excellence, University of Muenster, Muenster, Germany

   Contribution

   WH, Conception and design, Analysis and interpretation of data, Drafting or revising the article

   For correspondence

   wiebke.herzog@mpi-muenster.mpg.de

   Competing interests

   The authors declare that no competing interests exist.

• 2,990

  views

• 796

  downloads

• 89

  citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.