Synaptic activity regulates AMPA receptor trafficking through different recycling pathways

  1. Ning Zheng
  2. Okunola Jeyifous  Is a corresponding author
  3. Charlotte Munro
  4. Johanna M Montgomery
  5. William N Green
  1. University of Chicago, United States
  2. University of Auckland, New Zealand

Abstract

Changes in glutamatergic synaptic strength in brain are dependent on AMPA-type glutamate receptor (AMPAR) recycling, which is assumed to occur through a single local pathway. Here we present evidence that AMPAR recycling occurs through different pathways regulated by synaptic activity. Without synaptic stimulation, most AMPARs recycled in dynamin-independent endosomes containing the GTPase, Arf6. Few AMPARs recycled in dynamin-dependent endosomes labeled by transferrin receptors (TfRs). AMPAR recycling was blocked by alterations in the GTPase, TC10, which co-localized with Arf6 endosomes. TC10 mutants that reduced AMPAR recycling had no effect on increased AMPAR levels with long-term potentiation (LTP) and little effect on decreased AMPAR levels with long-term depression. However, internalized AMPAR levels in TfR-containing recycling endosomes increased after LTP, indicating increased AMPAR recycling through the dynamin-dependent pathway with synaptic plasticity. LTP-induced AMPAR endocytosis is inconsistent with local recycling as a source of increased surface receptors, suggesting AMPARs are trafficked from other sites.

Article and author information

Author details

  1. Ning Zheng

    Department of Neurobiology, University of Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Okunola Jeyifous

    Department of Neurobiology, University of Chicago, Chicago, United States
    For correspondence
    ojeyifou@bsd.uchicago.edu
    Competing interests
    The authors declare that no competing interests exist.
  3. Charlotte Munro

    Department of Physiology, University of Auckland, Auckland, New Zealand
    Competing interests
    The authors declare that no competing interests exist.
  4. Johanna M Montgomery

    Department of Physiology, University of Auckland, Auckland, New Zealand
    Competing interests
    The authors declare that no competing interests exist.
  5. William N Green

    Department of Neurobiology, University of Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.

Ethics

Animal experimentation: We followed AVMA guidelines to prevent pain and suffering of animals, and only used the minimum number of animals necessary to obtain conclusions in our experiments. Any pain, discomfort, or distress associated with the surgical procedures was prevented by the administration of the volatile anesthetic, Isoflurane. All animal procedures have been approved by the University of Chicago Institutional Animal Care and Use Committee (IACUC/ACUP protocol #72016) and are in accordance with the recommendations of the Panel on Euthanasia of the American Veterinary Medical Association.

Reviewing Editor

  1. Reinhard Jahn, Max Planck Institute for Biophysical Chemistry, Germany

Publication history

  1. Received: February 5, 2015
  2. Accepted: May 13, 2015
  3. Accepted Manuscript published: May 13, 2015 (version 1)
  4. Version of Record published: June 2, 2015 (version 2)

Copyright

© 2015, Zheng et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Ning Zheng
  2. Okunola Jeyifous
  3. Charlotte Munro
  4. Johanna M Montgomery
  5. William N Green
(2015)
Synaptic activity regulates AMPA receptor trafficking through different recycling pathways
eLife 4:e06878.
https://doi.org/10.7554/eLife.06878

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