Representative whole-cell patch-clamp recordings and current–voltage relationships from (A) ANO6 and (B) ANO1 expressing cells with 200 μM [Ca2+]i. Currents were recorded in 150 mM or 15 mM extracellular CsCl. The reversal potentials (Erev) shift very little with ANO6-expressing cells, while the shift is large for ANO1-expressing cells. (C) Average Erev values for ANO6 or ANO1 expressing cells bathed in 146 NaCl, 150 CsCl, 15 NaCl, 15 CsCl, or 150 NMDG-Cl. (D) Relative permeabilities calculated from the Goldman-Hodgkin-Katz equation. N = 6–17.