Dispersed human islets were mock infected or infected with CVB5 (M.O.I. 10) for 8 hr. After infection, cells were fixed and used for histological studies. Fluorescent microscopy analysis of insulin (A and C, in green), glucagon (B and D, in cyan), and VP-1(E–H, in red) shows the presence of double-positive cells for insulin and VP-1 (O, merged panels, in yellow) and glucagon and VP-1(P, merged panels, in yellow/white) after CVB5 infection. No VP-1 positive cells (E and F) were observed in mock-infected cells. Nuclear staining was performed with Hoechst (I–L, in blue). Double-positive cells for insulin and VP-1 and for glucagon and VP-1 are indicated by the arrows (C, D, G, H, K, L, O, and P panels).