Examining kinesin processivity within a general gating framework
- Stanford University, United States
- Pennsylvania State University, United States
Figures
Figure 1
A general gating framework based on mechanical states of dimeric motors.
(A) The kinesin mechanochemical cycle. Kinesin starts from the one-head-bound (1-HB) ATP-waiting state [α], characterized by a strongly bound, nucleotide-free (Ø) front head (red) and an unbound, ADP-containing tethered head (blue). ATP binding induces a force-dependent transition involving partial NL docking, shifting the tethered head past the bound head [β1]. ATP hydrolysis completes NL docking and facilitates tethered-head binding [β2]. At this point, kinesin may access a dissociated state [Off], induced by premature phosphate release from the bound head, leading to dimer detachment. However, if the tethered head reaches the forward MT binding site and completes the step before the bound head can dissociate, kinesin enters the two-heads-bound (2-HB) state [γ]. Rear-head release returns the dimer to the ATP-waiting state [α], having moved forward by 8.2 nm. (B) A simplified general gating framework, based on the cycle in (A). Stepping, binding, and unbinding gates are shown with associated rate constants between each of the three gated states, [A], [B], and [C]. The cycle begins at the 1-HB ATP-waiting state [A], where the stepping gate promotes processivity by inhibiting rear-head (blue) rebinding and premature bound-head (red) release. Following a force-dependent step that shifts the tethered head past the bound head [B], the binding gate promotes binding of the tethered head at the forward MT binding site while inhibiting release of the bound head. Also shown is a competing dissociated state [Off], arising from premature release of the bound head from the 1-HB state, accessible from either [A] or [B]. Tethered-head binding leads to the 2-HB state [C], where the unbinding gate promotes rear-head release while inhibiting front-head release, returning the motor to the start of the cycle [A].
Figure 2
Kinesin with as many as 3 AA inserted in the NL maintains a 1-HB ATP-waiting state.
(A) Half-site mantADP release measurements as a function of NL insert length (mean ± SE; N = 3). Upon MT binding, both DmK-WT and DmK-3AA, pre-incubated with mantADP (mantD), lose ∼50% of their initial fluorescence. The fluorescence loss exceeds 50% for constructs containing NL inserts longer than 3 AA. Inset, A 50% loss of fluorescence corresponds to dimers binding to MTs in a 1-HB state, whereas a 100% fluorescence decrease is consistent with the release of all bound mantADP (mantD) upon MT binding. (B) MantADP exchange by the tethered head as a function of NL insert length (mean ± SE; N = 3), measured by rapidly diluting mantADP·kinesin·MT complexes into nucleotide-free buffer via stopped flow. The cartoon depicts the measured reaction. The exchange rate increased significantly for constructs with NL inserts of 4 AA or more. In the insets (A and B), white shading indicates non-fluorescent, nucleotide-free heads (Ø); yellow indicates fluorescent, mantADP-bound heads.
Figure 3
Extending the NL by a single AA compromises processivity.
Mean run lengths as a function of applied force (mean ± SE; N = 49–818) for the constructs studied, acquired with an optical force clamp in the presence of 2 mM ATP (solid circles; color-coded according to the legend). DmK-WT (Milic et al., 2014) and hindering load data sets for DmK-3AA (Andreasson et al., 2015) are reproduced from our previous work. For all constructs, mean run lengths exhibited significant asymmetry, depending upon the direction of load. To obtain the unloaded run length (L0) and characteristic distance parameter (δL) for each construct, run length (L) data for hindering (−6 to 0 pN) and assisting loads (+2 to +20 pN) were separately fit to exponentials (solid lines; color-coded according to the legend) of the form , where F is the force applied by the optical trap and kBT is Boltzmann's constant times the absolute temperature; parameter values are in Table 1. Inset cartoon, a graphical representation of the experimental geometry of the single-molecule assay (not to scale).
Figure 4
Added phosphate enhances the processivity of DmK-WT and DmK-6AA.
(A) Mean run lengths (mean ± SE; N = 84–210) under moderate assisting load (+4 pN), in the presence of nucleotide analogs (green), 100 mM potassium chloride (KCl; purple), or 100 mM potassium phosphate (KPi; orange). Run lengths for DmK-WT (shaded bars, data from Milic et al., 2014) are shown paired with DmK-6AA data (unshaded bars). Decreasing the ATP concentration, replacing ATP by ATPγS, or adding KCl elicited no statistically significant change in run length relative to the baseline run length for saturating ATP (2 mM) in the absence of added salt. The mean run length increased significantly in the presence of phosphate for both DmK-WT (p < 10−4; t-test) and DmK-6AA (p < 10−4; t-test). (B) Run-length data from (A), normalized to the baseline run length value for each construct.
Figure 5
Assisting load can rescue the velocity of mutants with extended NLs.
Velocity (mean ± SE; N = 49‒818) as a function of force for constructs (solid circles; color-coded according to the legend). Data were collected under the same conditions as Figure 3. DmK-WT velocity was not affected by assisting loads, but the velocities of all mutant constructs could be increased by larger assisting loads. Solid lines show the global fit to a minimal 3-state model (inset) for WT (blue) and mutant (red) constructs, with parameters in Table 2. Data sets for both DmK-WT and DmK-3AA under hindering loads are from (Andreasson et al., 2015). Force–velocity data are compared to other mutant constructs in Figure 7.
Figure 6
Kinesin unbinding rates are asymmetric with respect to the direction of load.
Single-molecule measurements of the rate of MT unbinding for DmK-WT (mean ± SE; N = 75‒818) at 2 mM ATP (solid circles). The unloaded release rate (koff) and the associated distance parameter (δoff) were obtained from exponential fits to unbinding data acquired under hindering loads (−), −25 to 0 pN, and assisting loads (+), +2 to +20 pN. Fits (solid lines) and associated parameters (legend; mean ± SE) are shown.
Figure 7
Kinesin motility characteristics depend upon parent species, NL length, NL sequence, and cysteine mutations.
Force–velocity relations of constructs that differ by parent species, NL length, NL insert sequence, or cysteine mutations (mean ± SE; N = 25–818; solid circles, color-coded according to the legends). (A) DmK-6AA exhibited lower velocity under all loads than WT, but was faster and less force-dependent than HsK-CL-6AA, a human CL construct with a different 6-AA NL insert. (B) Side-by-side comparison of human and Drosophila CL mutants, along with corresponding WT constructs. Unloaded velocities of CL constructs with WT-length NL domains were similar, but CL constructs were systematically slower than WT under hindering loads. Under assisting loads, only the HsK-CL construct could be sped up beyond WT velocities. (C and D) Comparisons of constructs carrying NL insert sequences LQASQT (C) and AEQKLT (D). Force–velocity data for WT Drosophila and human kinesin were indistinguishable, but human constructs with 6-AA extensions of the NL moved at lower velocities than corresponding Drosophila motors under all loads. (E and F) Comparisons of all Drosophila (E) and human (F) constructs. Constructs with the NL insert AEQKLT were slower for all forces than those with LQASQT, for both Drosophila and human kinesin. HsK-CL-6AA data (Clancy et al., 2011) and hindering-load velocities for DmK-WT (Andreasson et al., 2015) are reproduced from previous work. Fits to DmK-WT and DmK-6AA data sets (solid lines; color-coded according to the legends) correspond to the model of Figure 5, with parameters values from Table 2. The remaining force–velocity data were fit to polynomials (solid and dashed lines; color-coded according to legends), provided to guide the eye.
Tables
Table 1
Parameters from exponential fits to the run length data of Figure 3
| Construct | L0− (nm)* | δL− (nm)* | L0+ (nm)* | δL+ (nm)* |
|---|---|---|---|---|
| DmK-WT† | 1120 ± 60 | 2.0 ± 0.1 | 87 ± 6 | 0.27 ± 0.03 |
| DmK-1AA | 360 ± 30 | 1.6 ± 0.1 | 120 ± 7 | 0.48 ± 0.02 |
| DmK-2AA | 410 ± 60 | 1.8 ± 0.2 | 115 ± 17 | 0.42 ± 0.05 |
| DmK-3AA | 320 ± 20 | 1.6 ± 0.1 | 76 ± 4 | 0.31 ± 0.02 |
| DmK-5AA | 440 ± 60 | 2.4 ± 0.2 | 48 ± 14 | 0.35 ± 0.14 |
| DmK-6AA | 270 ± 30 | 1.9 ± 0.1 | 59 ± 8 | 0.27 ± 0.10 |
-
L0−, unloaded run length for hindering loads (−6 to 0 pN); δL−, distance parameter for hindering loads (−6 to 0 pN); L0+, unloaded run length for assisting loads (+2 to +20 pN); δL+, distance parameter for assisting loads (+2 to +20 pN).
-
*
Parameter values correspond to mean ± SE.
-
†
Values from Milic et al. (2014).
Table 2
Kinetic parameters from a global fit of the 3-state model to the force–velocity data of Figure 5
| Parameter | Parameter description | Value* |
|---|---|---|
| Rate of ATP binding†; mechanical step | 4900 ± 300 s−1 | |
| Distance parameter (wild-type) | 4.6 ± 0.1 nm | |
| Distance parameter (mutant constructs) | 4.0 ± 0.1 nm | |
| Rate of ATP hydrolysis; biochemical events | 95 ± 1 s−1 | |
| Rate of rear-head release | 260 ± 10 s−1 | |
| Distance parameter (rear-head release) | 0.35 ± 0.02 nm | |
| Inter-head tension (wild-type) | 26 ± 3 pN | |
| Inter-head tension (mutant constructs) | 0 pN (fixed) |
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*
Parameter values correspond to mean ± SE.
-
†
Rate for saturating ATP conditions (2 mM).
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Examining kinesin processivity within a general gating framework
eLife 4:e07403.
https://doi.org/10.7554/eLife.07403
