The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
- Warwick Medical School, United Kingdom
- University of Liverpool, United Kingdom
- University of Edinburgh, United Kingdom
Abstract
Kinetochore fibers (K-fibers) of the mitotic spindle are force-generating units that power chromosome movement during mitosis. K-fibers are composed of many microtubules that are held together throughout their length. Here we show, using 3D electron microscopy, that K-fiber microtubules are connected by a network of microtubule connectors. We term this network 'the mesh'. The K-fiber mesh is made of linked multipolar connectors. Each connector has up to four struts, so that a single connector can link up to four microtubules. Molecular manipulation of the mesh by overexpression of TACC3 causes disorganization of the K-fiber microtubules. Optimal stabilization of K-fibers by the mesh is required for normal progression through mitosis. We propose that the mesh stabilizes K-fibers by pulling MTs together and thereby maintaining the integrity of the fiber. Our work thus identifies the K-fiber meshwork of linked multipolar connectors as a key integrator and determinant of K-fiber structure and function.
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© 2015, Nixon et al.
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The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
eLife 4:e07635.
https://doi.org/10.7554/eLife.07635
