(A, B) In strains similar to those used in Figure 3B, TERT (Est2) was expressed from its endogenous locus bearing a C-terminal 13myc tag, separated by an 8-glycine linker. TLC1, TLC1Δ48, and TLC1(Ku)3 were expressed as in Figure 3B, but cells were not passaged after loss of the pTLC1-URA3 cover plasmid. Cells were crosslinked and subjected to chromatin immunoprecipitation (ChIP) using the myc epitopes on TERT, as described (Fisher et al., 2004). Telomeric enrichment was measured using quantitative real-time PCR (qPCR) amplicons close to telomere VI-R (A) and telomere XV-L (B). An amplicon at the ARO1 locus was used as a non-telomeric control locus. The thick horizontal lines on the graphs represent averages of three to five independent biological replicates, which themselves are indicated by black dots.