(A) Heterozygote and mutant Nfe2 pups at P1 were used to stain CD41 (platelets) and Shh. Dashed line was used to outline the DG. Embryonic dermis (skin) and blood streaks were used for Shh and CD41 staining. HF = a hair follicle in the skin. (A′) The ratio of CD41+;Shh + platelets to total Shh + cells was measured (n = 6). (B, C) Nfe2 embryos at E16.5 or pups at P1 were used to stain dentate progenitors (Ki67) and Pdgfrα+ meninges or Reelin + Cajal Retzius cells were co-stained to outline the embryonic DG. (B′) Numbers of dentate progenitors (Ki67 + or Lef1+) were plotted (n = 6). (D, D′) The Ki67 + dentate progenitors were stained at P3. The plot shows the decreased Ki67 + dentate progenitors in Nfe2 mutants (n = 6, D′). (E, E′) The Ptch1-LacZ + dentate progenitors were stained at P3 using Nfe2;Ptch1-LacZ pups. The plot shows the decreased Ptch1-LacZ + dentate progenitors in Nfe2 mutants (n = 3, E′). (F, F′) The dentate progenitors (Ki67, Lef1, Blbp) were stained at P5 when a few Nfe2 mutant survived. The plot shows the decreased dentate progenitors in the Nfe2 mutant (n = 3, F′). Dashed lines denote the outline of the DG. Student t-test was used to address the statistical significance. **, p < 0.05, ***, p < 0.001, ****, p < 0.0001. Scale bars: all = 200 μm except A (skin and blood streak) = 50 μm.