Percentages and absolute numbers of CD3+ and CD3+CD8+ cells in Healthy Donors (HD), Sustained Virologic Responder (SVR), and chronic HCV (cHCV) patients are provided in Figure 1—figure supplement 1 and 2. (A) Representative examples of CD45RA+CD27+ naïve CD8+ T cell compartment in the three donor subsets. FACS plots are gated on Live CD3+CD8+ cells. Validation of CD45RA/CD27 gating strategy for identifying naïve CD8+ T cells in cHCV patients is provided in Figure 1—figure supplement 3. (B) Percentages of naïve CD8+ T cells in the three donor subsets. (C) Absolute numbers (G/L) of naïve CD8+ T cells in HD, SVR, and cHCV patients. ns (not significant, p>0.05), *(p≤0.05), **(p≤0.01), and ***(p≤0.001) refer to Dunn’s multiple comparison test of each subset toward HD. (D) Normalized numbers of sjTRECs per 150,000 naïve CD8+ in HD and cHCV samples. Normalized numbers of sjTRECs per total CD8+ T cells are provided in Figure 1—figure supplement 4. (E) Representative example of the distribution of 24 FACS-screened Vβ families in naïve CD8+ T cells from one HD and one cHCV sample. Families are ordered by increasing size in both individuals. (F) Lorenz curves representing the cumulative distribution of % of usage of 24 FACS-screened Vβ families from 7 HD and 7 cHCV patients. Mean Gini coefficients and standard deviations are indicated. Red line indicates an extreme example of an unequal distribution, observed in the case of a T-cell lymphoma where >90% of the TCR repertoire is explained by one particular Vβ chain. (G) Individual Gini coefficients of all tested samples are represented for HD and cHCV subgroups.