Cell-to-cell infection by HIV contributes over half of virus infection

  1. Shingo Iwami  Is a corresponding author
  2. Junko S Takeuchi
  3. Shinji Nakaoka
  4. Fabrizio Mammano
  5. François Clavel
  6. Hisashi Inaba
  7. Tomoko Kobayashi
  8. Naoko Misawa
  9. Kazuyuki Aihara
  10. Yoshio Koyanagi
  11. Kei Sato  Is a corresponding author
  1. Kyushu University, Japan
  2. Japan Science and Technology Agency, Japan
  3. Kyoto University, Japan
  4. University of Tokyo, Japan
  5. Hospital Saint Louis, France
  6. Université Paris Diderot, Sorbonne Paris Cité, France
  7. Tokyo University of Agriculture, Japan
5 figures, 2 tables and 3 additional files

Figures

Cell culture systems and the basic reproduction number under cell-to-cell and cell-free infection.

(A) Static and shaking cultures of Jurkat cells. The static and shaking cell cultures allow human immunodeficiency virus type 1 (HIV-1) to perform both cell-free and cell-to-cell infection, and only …

https://doi.org/10.7554/eLife.08150.003
Figure 2 with 3 supplements
Dynamics of HIV-1 infection in Jurkat cells through cell-free and cell-to-cell infection.

Jurkat cells were inoculated with HIV-1 (at multiplicity of infection 0.1) in the static and shaking cell cultures. Panels A and B show the time-course of experimental data for the numbers of …

https://doi.org/10.7554/eLife.08150.005
Figure 2—figure supplement 1
No effect of the shaking procedure on HIV-1 cell-free infection.

Jurkat cells were infected with HIV-1 (at multiplicity of infection 1) as described in ‘Materials and methods’, and the infected cells were cultured in the static and the shaking condition. By …

https://doi.org/10.7554/eLife.08150.006
Figure 2—figure supplement 2
Dynamics of Jurkat cell growth.

Dynamics of Jurkat cell growth in the static and shaking cell cultures. By harvesting the cells for 37 days (A) in the static and (B) in the shaking cell cultures, the growth kinetics of Jurkat …

https://doi.org/10.7554/eLife.08150.007
Figure 2—figure supplement 3
Dot plots of infected cells by flow cytometry.

Representative results of flow cytometry (experiment 1). Time-course results of flow cytometry analyses on experiment 1 of static (left) and shaking (right) cultures are respectively shown. The …

https://doi.org/10.7554/eLife.08150.008
Distribution of the basic reproduction numbers, generation time, and Malthus coefficient.

The distribution of the basic reproduction number, R0, the number of secondary infected cells through the cell-free infection, Rcf, and the cell-to-cell infection, Rcc, calculated from all accepted …

https://doi.org/10.7554/eLife.08150.009
Simulating cell-to-cell infection of HIV-1.

Using our estimated parameters, the pure cell-to-cell infection is simulated in silico (solid curves). The simulated values are located between the time course of experimental data under the static …

https://doi.org/10.7554/eLife.08150.011
Author response image 1
Punctual model for parameter estimation.

The time course of experimental data for the numbers of uninfected cells (top) and infected cells (middle), and the amount of viral p24 antigen (bottom) in the static (left) and shaking (right) …

https://doi.org/10.7554/eLife.08150.017

Tables

Table 1

Parameters estimated by mathematical-experimental analysis

https://doi.org/10.7554/eLife.08150.004
Parameter nameSymbolUnitExp. 1Exp. 2Exp. 3Ave. ± S.D.
Parameters obtained from simultaneous fit to time-course experimental dataset
 Rate constant for cell-free infectionβ10−6 × (p24 day)−15.59* (3.54–8.41)3.27 (2.05–5.01)3.70 (2.28–5.77)4.18 ± 1.41
 Rate constant for cell-to-cell infectionω10−6 × (cell day)−10.88 (0.45–1.39)1.25 (0.70–1.97)1.13 (0.64–1.79)1.09 ± 0.33
 Production rate of total viral proteinpday−10.37 (0.22–0.59)0.59 (0.34–0.92)0.54 (0.31–0.86)0.50 ± 0.16
 Death rate of infected cellsδday−10.45 (0.32–0.64)0.54 (0.38–0.75)0.50 (0.36–0.68)0.50 ± 0.10
Quantities derived from fitted values
 Basic reproduction number through cell-free infectionRcf2.88 (2.34–3.53)2.27 (1.98–2.66)2.43 (2.04–2.95)2.44 ± 0.23
 Basic reproduction number through cell-to-cell infectionRcc2.95 (1.48–4.70)3.65 (1.77–6.05)3.39 (1.82–5.38)3.39 ± 0.91
 Basic reproduction numberR05.83 (4.20–7.75)5.92 (3.99–8.46)5.83 (4.21–7.89)5.83 ± 0.94
 Contribution of cell-to-cell infectionRccRcf+Rcc0.50 (0.34–0.63)0.60 (0.44–0.72)0.57 (0.43–0.70)0.57 ± 0.07
  1. *

    Mean value.

  2. 95% confidence interval.

  3. Average and standard deviation of merged values in experiment 1, 2, and 3.

Table 2

Generation time and Malthus coefficient of virus infection

https://doi.org/10.7554/eLife.08150.010
Cell culture systemExp. 1Exp. 2Exp. 3Ave. ± S.D.
Generation time of HIV-1
 Static cell culture2.51* days2.08 days2.22 days(2.22 ± 0.32) days
(1.78–3.38) days(1.54–2.78) days(1.69–2.93) days
 Shaking cell culture2.73 days2.34 days2.47 days(2.47 ± 0.32) days
(1.99–3.59) days(1.77–3.06) days(1.91–3.18) days
Malthus coefficient of HIV-1
 Static cell culture1.61 day−12.03 day−11.86 day−1(1.86 ± 0.37) day−1
(1.10–2.27) day−1(1.32–3.01) day−1(1.26–2.72) day−1
 Shaking cell culture0.57 day−10.46 day−110.49 day−1(0.49 ± 0.05) day−1
(0.47–0.67) day−1(0.38–0.56) day−1(0.39–0.61) day−1
  1. *

    Mean value.

  2. 95% confidence interval.

  3. Average and standard deviation of merged values in experiment 1, 2, and 3.

  4. HIV-1, human immunodeficiency virus type 1.

Additional files

Supplementary file 1

Technical details of MCMC computations.

https://doi.org/10.7554/eLife.08150.012
Supplementary file 2

Estimated parameter values for Jurkat cell growth.

https://doi.org/10.7554/eLife.08150.013
Supplementary file 3

Estimated initial values for HIV-1 infection.

https://doi.org/10.7554/eLife.08150.014

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