The vacuole/lysosome is required for cell-cycle progression

  1. Yui Jin
  2. Lois S Weisman  Is a corresponding author
  1. University of Michigan, United States

Abstract

Organelles are distributed to daughter cells, via inheritance pathways. However, it is unclear whether there are mechanisms beyond inheritance, which ensure that organelles are present in all cells. Here we present the unexpected finding that the yeast vacuole plays a positive essential role in initiation of the cell-cycle. When inheritance fails, a new vacuole is generated. We show that this occurs prior to the next cell-cycle, and gain insight into this alternative pathway. Moreover, we find that a combination of a defect in inheritance with an acute block in the vacuole biogenesis results in the loss of a functional vacuole and a specific arrest of cells in early G1 phase. Furthermore, this role for the vacuole in cell-cycle progression requires an intact TORC1-SCH9 pathway that can only signal from a mature vacuole. These mechanisms may serve as a checkpoint for the presence of the vacuole/lysosome.

Article and author information

Author details

  1. Yui Jin

    Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Lois S Weisman

    Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, United States
    For correspondence
    lweisman@umich.edu
    Competing interests
    The authors declare that no competing interests exist.

Copyright

© 2015, Jin & Weisman

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 5,913
    views
  • 1,032
    downloads
  • 56
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Citations by DOI

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Yui Jin
  2. Lois S Weisman
(2015)
The vacuole/lysosome is required for cell-cycle progression
eLife 4:e08160.
https://doi.org/10.7554/eLife.08160

Share this article

https://doi.org/10.7554/eLife.08160