(A–E) Stage 17 Drosophila embryos of the indicated genotypes stained with anti-HRP antibodies to label all CNS axons. (F–J) Stage 15–16 embryos of the indicated genotypes carrying eg-GAL4 and UAS-Tau…
(A–E) Stage 17 embryos carrying elav-GAL4 and the indicated UAS-Robo transgenes, stained with anti-HRP (magenta) and the longitudinal pathway marker anti-FasciclinII (FasII; green). (A) Embryos …
Since the effects of expressing ∆C transgenes in the 86Fb insertion site are too potent to allow quantitative comparison, we used traditional UAS insertion lines that are expressed at lower and …
(A–E) Slit-conditioned media was collected and used to treat cells expressing the indicated HA-tagged receptors. Receptor expression is shown with anti-HA in the top panels (magenta) and anti-Slit …
Fluorescence intensities are normalized to the average pixel intensity of untransfected cells from the same slide. The values from three images per Robo2 variant were averaged; error bars represent …
(A–E) Embryos carrying elav-GAL4 and the indicated UAS-Robo2 transgenes were stained with anti-HA antibodies and imaged via confocal microscopy. Staining and imaging conditions were identical for …
(A–F) Stage 17 embryos carrying elav-GAL4 and the indicated UAS-Robo transgenes, stained with anti-HRP and anti-FasII. (A) Embryos carrying elav-GAL4 alone exhibit a wild-type arrangement of axon …
(A) Stage 17 embryos stained with anti-HRP (magenta) and anti-GFP (green) antibodies. Anti-GFP labels the apterous (ap) cell bodies and axons, which normally project ipsilaterally. Mis-expression of …
(A–D) Stage 17 embryos stained with anti-HRP (magenta) and anti-FasII (green). (A and B) Mis-expression of Robo1 (A) in midline cells using slit-GAL4 results in a mild ectopic crossing phenotype. In …
(A) A robo2-GAL4 enhancer trap that recapitulates robo2's endogenous expression pattern drives UAS-TauMycGFP expression (green) in midline cells at stages 12–13, when many commissural axons cross …
Fluorescent in situ for robo2 mRNA (green). robo2 is transiently expressed in midline glia (magenta) during Stages 12 and 13 but is no longer detected there by Stage 14. Midline glia are labeled …
(A–D) Stage 16 embryos of the indicated genotypes stained with anti-HRP (magenta) and anti-GFP (green) antibodies. Anti-GFP labels the EG and EW cell bodies and axons. EW crossing defects in robo2, f…
(A) Schematic diagram of the two chimeric receptors shown in (B and C). Robo1 sequences are depicted in blue and Robo2 sequences are depicted in yellow. (B and C) Stage 16 embryos of the indicated …
(A–C) Protein extracts from embryos expressing Robo1-Myc and various HA-tagged receptors in all neurons were immunoprecipitated with anti-Myc antibodies and analyzed by western blot. …
(A–C) Protein extracts from embryos expressing Robo1-Myc and various HA-tagged receptors in all neurons were immunoprecipitated with anti-Myc antibodies and analyzed by western blot. …
(A–D) Stage 16 embryos stained with anti-HRP (magenta) and anti-GFP (green) antibodies. Anti-GFP labels the EG and EW cell bodies and axons. Arrowheads indicate EW axons that have crossed the …
In contralateral neurons, the endosomal sorting receptor Comm is expressed, and it prevents the majority of Robo1 from reaching the growth cone surface. We propose that Robo2 acts non-autonomously …