Developmental compartments in the larval trachea of Drosophila
- University of California, San Francisco, United States
Figures
Figure 1
Cell divisions in the second tracheal metamere during the third instar.
(A) Drawings of the Tr2 branches before (early L3) and after (late L3) the onset of cell divisions; dorsal trunk (DT), dorsal branch (DB), visceral branch (VB), spiracular branch (SB), transverse …
Figure 2
Patterns of gene expression in the second tracheal metamere of third instar larvae.
(A-–C) A Tr2 preparation stained for Sal, Ser and Delta; (A) Sal expression was specific to the DT; (A’) anti-Sal antibody stained the DT, but not DB, TC or VB; (B) Ser was expressed in the DT, ASP …
Figure 3
Discrete regions of Notch activation in the second tracheal metamere.
(A) Drawing of a late L3 Tr2 metamere with red areas indicating the regions that express the Notch reporter NRE-lacZ; (B) Tr2 of a stage 14–16 embryo with tracheal nuclei marked with GFP …
Figure 4 with 7 supplements
Marked clones that meet but do not cross into or from the dorsal trunk.
(A-–D) Marked clones in the DT line the DT/DB junction. (A) large patch of GFP-expressing cells abuts the DT/DB junction; Cut expression in TC (red); clone induction by regimen Experiment E (Table 1)…
Figure 4—figure supplement 1
Dorsal trunk clones at the DT/DB border.
GFP-expressing clones induced by regimens A-H; arrows point to DT/DB junction. (A, B) NRE-LacZ (red); (C, H4,5) DAPI (blue); (D) anti-Delta (red); (E) anti-Cut (red); (F) anti-Ser (red); the …
Figure 4—figure supplement 2
Dorsal branch clones at the DT/DB border.
GFP-expressing clones induced by regimens B-H; arrows point to DT/DB junction. (B) NRE-LacZ (red); (C) DAPI (blue); (D) anti-Delta (red); (E) anti-Cut (red); (G, H) Dual clone regimens produced …
Figure 4—figure supplement 3
Dorsal trunk and dorsal branch clones abutting the same DT/DB border.
GFP- and LacZ-expressing clones induced by regimen G; arrows point to DT/DB junction. Regimen G produced clones that expressed GFP (green), LacZ (red) or both (yellow).
Figure 4—figure supplement 4
Dorsal trunk clones at the DT/TC border.
GFP-expressing clones induced by regimens A-H; arrows point to DT/TC junction. (A, B) NRE-LacZ (red); (C) DAPI (blue); (D) anti-Delta (red); (E) anti-Cut (red); (F) anti-Ser (red); (F1, F1”) images …
Figure 4—figure supplement 5
Transverse connective clones abutting the DT/TC border.
GFP-expressing clones induced by regimens C, D, E, F, H; arrows point to DT/TC junction. DAPI (blue); (D) anti-Delta (red); (E) anti-Cut (red); (F) anti-Ser (red); (H) Dual clone regimen produced …
Figure 4—figure supplement 6
Large marked cells in the TC domain of the DT.
Panel labels refer to marking regimens for clones; arrows indicate the one or two large marked cells in the TC domain and (*) indicates node cells. (A) NRE-LacZ (red); (D) anti-Delta (red); (E) …
Figure 4—figure supplement 7
Marked patches of cells that straddle the DT/DB and DT/TC borders.
Panel labels refer to marking regimens for DT/DB clones (upper panels) and DT/TC clones (lower panels); borders indicated by arrows. (A, B) NRE-LacZ (red); (D) anti-Delta (red); (E) anti-Cut (red); …
Figure 5
Mutant clones transform dorsal branch, dorsal trunk and transverse connective cells.
(A) Control DT clone of GFP-expressing cells with no effect on morphology or expression of Delta; (B-–F’) Clones of sal mutant DT cells generated bulges, apparently sorting out, and lacked Delta …
Figure 6
Notch signaling is required by dorsal branch cells at the junction with the dorsal trunk.
(A) Clone of DB cells that ectopically expressed Delta (green) increased the diameter of the DB and induced wild type neighbors to express vein-lacZ (red), normally expressed in the DB only by cells …
Tables
Table 1
Regimen | Marking system | # Specimens | # Marked DT | HS stage | HS | DT/DB border clones | DT/TC border clones | |||
DT side | DB side | DT DB | DT side | TC side | ||||||
A | GFP, NRE lacz | 106 | 40 | 48-50h AEL | 8' 35° | 3 | 0 | 5 | 0 | |
B | GFP, NRE-lacz | 59 | 24 | 24-32h AEL | 5' 37° | 7 | 1 | 8 | 0 | |
C | GFP | 29 | 11 | 24-32h AEL | 5' 37° | 2 | 1 | 1 | 1 | |
D | MARCM, α-Delta | 52 | 43 | 4-6h AEL | 60' 38° | 1 | 3 | 4 | 5 | |
E | MARCM, α-Cut | 48 | 34 | 4-6h AEL | 60' 38° | 6 | 2 | 6 | 5 | |
F | M MARCM, α-Ser | 251 | 30 | 4-6h AEL | 30' 38° | 3 | 2 | 2 | 3 | |
G | GFP & LacZ, α-Ser | 119 | 114 (GFP) 79 (LacZ)* | 24-26h AEL | 15' 37° | 11 | 11 | 6** | 8 | 0 |
H | GFP & LacZ | 116 | 61 (GFP) 34 (LacZ)* | 24-26h AEL | 6' 37° | 9 | 5 | 0 | 6 | 1 |
TOTAL | 780 | 361 | 42 | 25 | 6 | 40 | 15 | |||
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Genotype A: NRElacz/hsFLP; actin>y >GAL4,UAS-GFP/ ; /MKRS B: NRElacz/hsFLP; actin>y >GAL4,UAS-GFP/ ; /MKRS C: hsFLP/Y; actin>y >GAL4,UAS-GFP/ ; /MKRS D: hsFLP122,tubGAL4,UAS-NLS-GFP/ ; tubGal80 FRT40a/FRT40a; / E: hsFLP122,tubGAL4,UAS-NLS-GFP/ ;tubGal80,FRT40a /FRT40a; / F: hsFLP,tubGAL4, UAS-GFP-NLS; / ;RpS17,tubGal80,FRT80a/FRT80a G: hsFLP/ orY; actin>y >GAL4,UAS-GFP/ ;actin>stop>lacZ-NLS/MKRS H: hsFLP/ orY; actin>y >GAL4,UAS-GFP/ ;actin>stop>lacZ-NLS/MKRS * # DTs with clones at or near the DB and TC borders ** specimens with independently marked DT and DB clones
