(A) Schematic illustrations of a new EmrE mutant with elongated, 10-bead loops, and a new CB mutant with shortened, 5-bead loops. (B) Fraction of CG trajectories that reach fully integrated topologies as a function of time for the EmrE and CB mutants with both loop lengths. The integration kinetics for the EmrE mutants clearly show that longer loops increase the timescale of post-translational annealing, due to the reduction of the loop-flipping frequency for the longer soluble loops. The corresponding effect is less clear for the two CB mutants, since the timescale for the loop-flipping frequency is dominated by the large positive charges on these loops, masking the effect of changing the loop length. (C) Average loop positions in the end-of-translation (EOT) ensemble for both the EmrE and CB mutants, presented in terms of the fraction of configurations for which each loop occupies the cytoplasm. For the EmrE mutants, changing the loop length leads to relatively small shifts in the EOT ensemble; however, for the CB mutants, the effect of loop length on the EOT ensemble is much larger, with the new short-loop CB mutant exhibiting a much weaker bias towards the Ncyto/Ccyto topology in the EOT ensemble.