(A) Strand-specific RNA-seq data from the two biological replicates of wild type strain (JKM139) before (time zero, T0) and 60 (T60) or 240 (T240) min after HO induction, were uniquely mapped to the MAT locus ±10 kb. For each time point, densities (tag/nucleotide, log2 scale) from the two replicates were pooled and visualized as a heatmap with the upper and lower panels corresponding to the + and − strands, respectively. Black arrows represent annotated genes. (B) Density for genes along the MAT locus ±10 kb at T0, T60 and T240 after HO induction. Mean values ±s.d. were calculated from the two biological replicates analyzed. RRT12 showed too low signal to be significantly quantified and was not included. (C) Ratio of density for genes along the MAT locus ±10 kb at T0, T60 and T240 after HO induction. For each time point, densities were normalized on the values obtained at T0. Mean values ±s.d. were calculated as above. RNA-seq data used to construct the graphs of Figure 1B,C are available in Figure 1—source data 1.