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NG2 glia are required for vessel network formation during embryonic development

  1. Shilpi Minocha
  2. Delphine Valloton
  3. Isabelle Brunet
  4. Anne Eichmann
  5. Jean-Pierre Hornung
  6. Cecile Lebrand  Is a corresponding author
  1. University of Lausanne, Switzerland
  2. Institut national de la santé et de la recherche médicale, Collège de France, France
Research Article
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Cite this article as: eLife 2015;4:e09102 doi: 10.7554/eLife.09102

Abstract

The NG2+ glia, also known as polydendrocytes or oligodendrocyte precursor cells, represent a new entity among glial cell populations in the central nervous system. However, the complete repertoire of their roles is not yet identified. The embryonic NG2+ glia originate from the Nkx2.1+ progenitors of the ventral telencephalon. Our analysis unravels that, beginning from E12.5 until E16.5, the NG2+ glia populate the entire dorsal telencephalon. Interestingly, their appearance temporally coincides with the establishment of blood vessel network in the embryonic brain. NG2+ glia are closely apposed to developing cerebral vessels by being either positioned at the sprouting tip cells or tethered along the vessel walls. Absence of NG2+ glia drastically affects the vascular development leading to severe reduction of ramifications and connections by E18.5. By revealing a novel and fundamental role for NG2+ glia, our study brings new perspectives to mechanisms underlying proper vessels network formation in embryonic brains.

Article and author information

Author details

  1. Shilpi Minocha

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  2. Delphine Valloton

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  3. Isabelle Brunet

    Institut national de la santé et de la recherche médicale, Collège de France, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  4. Anne Eichmann

    Institut national de la santé et de la recherche médicale, Collège de France, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  5. Jean-Pierre Hornung

    Department of Fundamental Neurosciences, University of Lausanne, Lausnne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  6. Cecile Lebrand

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    For correspondence
    cecile.lebrand@unil.ch
    Competing interests
    The authors declare that no competing interests exist.

Ethics

Animal experimentation: All studies on mice of either sex have been performed in compliance with the national and international guidelines and with the approval of the Federation of Swiss cantonal Veterinary Officers (2164).

Reviewing Editor

  1. Joseph G Gleeson, Howard Hughes Medical Institute, The Rockefeller University, United States

Publication history

  1. Received: June 1, 2015
  2. Accepted: November 29, 2015
  3. Accepted Manuscript published: December 10, 2015 (version 1)
  4. Accepted Manuscript updated: January 5, 2016 (version 2)
  5. Version of Record published: February 11, 2016 (version 3)

Copyright

© 2015, Minocha et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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    Funding: This work was supported in part by the Italian Ministry of Health (GR-2013-02355724 and Ricerca Corrente), MJFF, ALZ, Alzheimer's Research UK and the Weston Brain Institute (BAND2015), and Euronanomed III (Speedy) to FM; by the Spanish Ministerio de Economía y Competitividad [grant AGL2016-78054-R (AEI/FEDER, UE)] to J.M.T. and J.C.E.; A.M.-M. was supported by a fellowship from the INIA (FPI-SGIT-2015-02).

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