(A,B,C and D) The dynamics of RelA-dsRedxp following 10 ng/ml TNFα treatment in transiently transfected SK-N-AS (A), or following 30 pg/ml TNFα treatment in SK-N-AS, and 10 ng/ml TNFα treatment in HeLa cells (C), and at 30 pg/ml for HeLa (D) cells (n=30 cells analysed per condition). (E) The localization of endogenous RelA in different cell cycle phases, observed by immunocytochemistry at 2 hr (G1/S transition), 4 hr (mid S-phase), post-release from double thymidine block and with 15 min TNFα treatment. (F and G) The dynamics of RelA-dsRedxp in transiently transfected HeLa cells synchronized by a double thymidine block, following 10 ng/ml TNFα treatment at G1/S (F), or passing through S-phase (G) (n=20 cells analysed per condition). (H) Western blot of Ser536phopho-RelA (p-RelA), IκBα, and cyclophilin-A (cyclo-A) levels in synchronized HeLa cells harvested at 1 hr time intervals over the G1/S transition following 15 min treatment with TNFα. Also shown are asynchronous, non-stimulated (ASY NST) and asynchronous, stimulated (ASY ST) controls, harvested at t=0.