(A) Wild-type (WT) or acentrosomal (p53-/-; SAS-6 -/-; clone #1) RPE1 cells were stained with anti-centrin (green) and γ-tubulin (red). DNA (DAPI, blue). (B) Western blot analysis of WT or SAS-6-/- …
(A, B) Sequence analyses of SAS-6 and p53 alleles in clone #1 and clone #2 as indicated. The sequence of wild-type and edited alleles are in black and blue, respectively. The lesion (deletion or …
(A, B) Wild-type (WT) or clone #2 acentrosomal (p53-/-; SAS-6 -/-) RPE1 cells were stained with indicated antibodies during interphase (A) and mitosis (B). DNA (DAPI, blue). (C, D) Clone #2 …
(A) Isogenic SAS-6-/-; p53-/- cells carrying indicated SAS-6 constructs (see ‘Methods’) were arrested in S phase and induced to express indicated SAS-6 mutants for 16 hr and then immunostained with …
p53-/-; SAS-6 -/- cells (clone #1) exogenously expressing full-length SAS-6 (FL) or various SAS-6 mutants as indicated were analyzed by immunofluorescence with indicated antibodies.
Isogenic SAS-6-/-; p53-/- cells carrying indicated SAS-6 constructs (see ‘Methods’) were induced to express indicated SAS-6 mutants for 3 days, and then processed for serial sectioning electron …
(A–C) Isogenic SAS-6-/-; p53-/- cells carrying indicated SAS-6 constructs (see ‘Methods’) were induced to express indicated SAS-6 mutants for 3 days, and then processed for serial sectioning …
Note that a centriole on the right in cross-sectional views contains nine MT triplets.
Note that a centriole on the right in cross-sectional views contains eight MT triplets.