De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly
- National Yang-Ming University, Taiwan
- The Rockefeller University, United States
- Academia Sinica, Taiwan
- Memorial Sloan-Kettering Cancer Center, United States
Abstract
Vertebrate centrioles normally propagate through duplication, but in the absence of preexisting centrioles, de novo synthesis can occur. Consistently, centriole formation is thought to strictly rely on self-assembly, involving self-oligomerization of the centriolar protein SAS-6. Here, through reconstitution of de novo synthesis in human cells, we surprisingly found that normal looking centrioles capable of duplication and ciliation can arise in the absence of SAS-6 self-oligomerization. Moreover, whereas canonically duplicated centrioles always form correctly, de novo centrioles are prone to structural errors, even in the presence of SAS-6 self-oligomerization. These results indicate that centriole biogenesis does not strictly depend on SAS-6 self-assembly, and may require preexisting centrioles to ensure structural accuracy, fundamentally deviating from the current paradigm.
Article and author information
Author details
Reviewing Editor
- Tim Stearns, Stanford University, United States
Version history
- Received: August 5, 2015
- Accepted: November 25, 2015
- Accepted Manuscript published: November 26, 2015 (version 1)
- Version of Record published: December 31, 2015 (version 2)
Copyright
© 2015, Wang et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly
eLife 4:e10586.
https://doi.org/10.7554/eLife.10586
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