Cell-cycle quiescence maintains Caenorhabditis elegans germline stem cells independent of GLP-1/Notch

This comma-separated value file contains counts of M-phase cells per gonadal arm for starvation, re-feeding, and control time courses of wildtype animals (Figure 2 and Figure 2—figure supplement 1). Each row of the file represents a single gonadal arm. Descriptors include treatment group (starvation, re-feeding, mock starvation, continued feeding), animal sex (hermaphrodite, male), age at the start of treatment (L4, early adult, L4 + 24 hr), replicate, and time.

This comma-separated value file contains counts of progenitor-zone cells in each phase of the cell cycle for starvation time courses of wildtype animals (Figure 3). Each row of the file represents a single cell. Descriptors include cell-cycle phase (G1, S-phase, G2, M-phase), animal sex (hermaphrodite, male), age at the start of starvation (early L4, mid L4, early adult), time, and a gonad identifier.

This comma-separated value file contains counts of progenitor-zone cells in each phase of the cell cycle for fed early adult hermaphrodites and fed hermaphrodites aged 24 hr post mid L4 (Figure 3—figure supplement 1A–B). Each set of four rows of the file represents a single progenitor zone. Descriptors include cell-cycle phase (G1, S-phase, G2, M-phase), age (early adult, L4 + 24h), and a gonad identifier.

This comma-separated value file contains counts of EdU-positive and EdU-negative M-phase cells in hermaphrodites exposed to EdU from the early adult stage or from 24 hr post mid L4 (Figure 3—figure supplement 1C). Each rows of the file represent a single time point. Descriptors indicate age at the start of EdU labeling (early adult, L4 + 24h).

This comma-separated value file contains propidium iodide intensities and nuclear volumes of interphase progenitor-zone nuclei and distal tip cell nuclei of six EdU-labeled gonadal arms (Figure 3—figure supplement 2). Gonadal arms are from fed early adult hermaphrodites or hermaphrodites starved from early adult for 3.5 hr. Each row of the file represents a single cell. Descriptors include treatment group (fed, starved), cell-cycle phase (G1, S-phase, G2, distal tip cell), and a gonad identifier.

This comma-separated value file contains counts of interphase and M-phase progenitor-zone cells for emb-30(tn377ts) hermaphrodites re-fed at the restrictive temperature or maintained in continued starvation (Figure 4). Each row of the file represents a single cell. Descriptors include treatment group (re-fed, starved), cell-cycle phase (interphase, M-phase), ranked proximity to the distal tip cell (numeric), time, and a gonad identifier.

This comma-separated value file contains counts of M-phase cells per gonadal arm for glp-1(oz112gf) and gld-3(q730) nos-3(q650) starvation and re-feeding time courses (Figure 5). Each row of the file represents a single gonadal arm. Descriptors include treatment group (starvation, re-feeding), genotype (glp-1(oz112gf), gld-3(q730) nos-3(q650)), and time.

This comma-separated value file contains measurements of the distance between the gonad distal tip and the first ‘crescent’ germ cell in temperature-shifted glp-1(q224) hermaphrodites (Figure 6A–F). Distances are measured in germ cell diameters. Each row of the file represents a single gonadal arm. Descriptors include growth conditions (fed, cdk-1 RNAi, starved from early adult, starved from mid-L4, 300 mM NaCl, no sperm) and temperature (15°C; 25°C for 8 hr; 25°C for 16 hr; 25°C for 24 hr; 25°C for 8 hr + 15°C for 2–3 days; 25°C for 16 hr + 15°C for 2–3 days; 25°C for 24 hr + 15°C for 2–3 days).

This comma-separated value file contains counts of M-phase cells and distances between the gonad distal tip and the first ‘crescent’ germ cell for two groups of animals: (i) glp-1(q224ts) hermaphrodites starved from early adult, shifted to 25°C for 8 hr, and then re-fed at 25°C, and (ii) glp-1(q224ts) hermaphrodites fed continuously and shifted to 25°C at the early adult stage (Figure 6G–H). Distances are measured in germ cell diameters. Each row of the file represents a single gonadal arm. Descriptors include treatment group (fed, starved) and time. NA, not determined.

This comma-separated value file contains counts of M-phase cells per gonadal arm for the starvation time courses shown in Figure 7B. Each row of the file represents a single gonadal arm. Descriptors include genotype (cep-1(gk138), aak-2(gt33), etc.) and time. For serotonin exposure, genotype is listed as ‘serotonin 0 hr’ or ‘serotonin 3 hr’.