(A) Collagen VII secretion was measured by western blotting and probing for collagen VII in RDEB/FB/C7 cell lysates and supernatants collected for 20 hr in the presence of ascorbic acid. In three …
(A) Het-1A cells transfected with siRNA as described in the methods section were incubated with ascorbate for 20h, fixed with methanol and immunostained with for collagen VII, HSP47 and TGN46. …
(A) Procollagen VII was accumulated in the ER of RDEB/FB/C7 cells that were either incubated at 15°C to accumulate secretory cargo in the ER and then released at 37°C for five minutes (control) or …
A schematic representation of human TANGO1 domain organization and its known interactors. SS, signal sequence; SH3, Src homology 3-like domain that is required for binding procollagen VII (Saito et …
(A) A scheme depicting the coding regions of the two constructs - TEER-Myc (residues 1177–1396 of TANGO1, tagged with Myc) and CC-TMCC3-Myc (residues 282–437 of TMCC3, tagged with Myc). HeLa cells …
(A) A scheme depicting the coding regions of the two constructs for mitochondrial targeting - Mit-TEER-Myc (residues 1187–1396 of TANGO1, including 11 amino acids of its transmembrane domain, tagged …
HeLa cells were seeded on coverslips and transfected with Mit-TEER-Myc or Mit-CC-TMCC3-Myc for 48 hr. For visualisation, cells were stained with DAPI (blue) and immunostained for Myc (green), and …
(A) For visualisation, cells were stained with DAPI (blue) and immunostained for Myc (green). The red channel shows immunostaining for ERGIC-53; YKT6 (after cytosol washout); early and late Golgi …
(A) Immunoblot of the parental HeLa cell line and two Tango1 CRISPR knock-out HeLa clones. Protein lysates were separated on a 6% SDS-PAGE and transferred onto a nitrocellulose membrane and probed …
(A) A pool of ERGIC membranes fuse to the ER exit site enriched in procollagen VII. This fusion is mediated by the t-SNARES BNIP1, Syntaxin18 and USE1; and the v-SNARE YKT6. The TEER domain of …