During the development, tight regulation of the expansion of neural progenitor cells (NPCs) and their differentiation into neurons is crucial for normal cortical formation and function. In this study, we demonstrate that microRNA (miR)-128 regulates the proliferation and differentiation of NPCs by repressing pericentriolar material 1 (PCM1). Specifically, overexpression of miR-128 reduced NPC proliferation but promoted NPC differentiation into neurons both in vivo and in vitro. In contrast, the reduction of endogenous miR-128 elicited the opposite effects. Overexpression of miR-128 suppressed the translation of PCM1, and knockdown of endogenous PCM1 phenocopied the observed effects of miR-128 overexpression. Furthermore, concomitant overexpression of PCM1 and miR-128 in NPCs rescued the phenotype associated with miR-128 overexpression, enhancing neurogenesis but inhibiting proliferation, both in vitro and in utero. Taken together, these results demonstrate a novel mechanism by which miR-128 regulates the proliferation and differentiation of NPCs in the developing neocortex.https://doi.org/10.7554/eLife.11324.001
The neurons that transmit information around the brain develop from cells called neural progenitor cells. These cells can either divide to form more progenitor cells or to become specific types of neurons. If these carefully regulated processes go wrong – for example, if progenitors fail to stop dividing in order to mature – a range of neurodevelopmental conditions may develop, including autism spectrum disorders.
Small RNA molecules called microRNAs control gene activity and protein formation by targeting certain other RNA molecules for destruction. One such microRNA, called miR-128, helps newly formed neurons to move to the correct region of the cortex – the outer layer of the brain, which is essential for many cognitive processes including thought and language. However, it was not clear whether miR-128 plays any other roles in the development of neurons.
Zhang, Kim et al. have now analysed the role of miR-128 in the developing cortex of mice. The findings suggest that miR-128 prevents cortical neural progenitor cells from dividing and supports their development into more specialized cells. Causing miR-128 to be over-produced in the progenitor cells caused the cells to divide less often and encouraged them to mature into neurons. Conversely, removing miR-128 from the progenitor cells caused them to divide more and resulted in fewer neurons forming.
Further investigation revealed that miR-128 works by causing less of a protein called PCM1 to be produced. Without this protein, cells cannot divide properly. Future studies could now investigate in more detail how miR-128 and PCM1 affect how the neurons in the cortex develop and work.https://doi.org/10.7554/eLife.11324.002
Neurogenesis, the process by which functionally integrated neurons are generated from neural progenitor cells (NPCs), involves the proliferation and neuronal fate specification of NPCs and the subsequent maturation and functional integration of the neuronal progeny into neuronal circuits (Gupta et al., 2002). Given its importance in the development of the nervous system, neurogenesis is tightly regulated at many levels by both extrinsic and intrinsic factors (Heng et al., 2010), and its disruption has been associated with various pathologies, including autism spectrum disorders (ASDs), Treacher Collins syndrome, and various neural tube defects (Sun and Hevner, 2014). Therefore, uncovering the molecular mechanisms that underlie neurogenesis is crucial to understand the functions and plasticity of brain development and to prevent such pathologies (Sun and Hevner, 2014).
MicroRNAs (miRNAs) are small noncoding RNA molecules that function in the transcriptional and post-transcriptional regulation of gene expression in a variety of organisms (Kawahara et al., 2012). Encoded by eukaryotic nuclear DNA, miRNAs function through imperfect base-pairing with complementary sequences in target mRNA molecules, typically triggering their targeted degradation or translational repression by the RNA-induced silencing complex RISC (Bartel, 2009; Shi et al., 2010). The role of miRNAs in neuronal development and function has recently received increased attention, and the specific spatiotemporal expression of these molecules may be essential for brain morphogenesis and neurogenesis (Volvert et al., 2012; Zhang et al., 2014). However, the specific miRNAs that regulate the proliferation and differentiation of NPCs during early cortical development are not well established.
In this study, we demonstrate that miR-128, which has previously been shown to play a crucial role in cortical migration (Franzoni et al., 2015), inhibits self-renewal and promotes neuronal differentiation in mouse NPCs by targeting pericentriolar material 1 (PCM1), a critical protein for cell division, during early cortical development (Ge et al., 2010). Specifically, ectopic overexpression of miR-128 reduces the proliferation of NPCs but promotes the differentiation of NPCs into neurons both in vivo and in vitro. Conversely, knockdown of miR-128 enhances proliferation but inhibits neuronal differentiation. Knockdown of endogenous PCM1 mimics the cellular phenotype of miR-128-overexpressing NPCs. Furthermore, the concomitant overexpression of both PCM1 and miR-128 in NPCs rescues the cellular phenotype associated with the overexpression of miR-128 in NPCs, indicating that PCM1 lies downstream of miR-128 and regulates the proliferation and neural fate specification of NPCs in vitro and in utero.
Taken together, our data indicate that miR-128 is an important regulator of neurogenesis in the embryonic cortex and suggest that aberrant miR-128 expression may account for the abnormal cortical development that underlies the pathophysiology of certain neuropsychiatric disorders, including autism.
To determine the spatial distribution of miR-128 in the developing embryonic cortex, we performed in situ hybridization (ISH) using digoxigenin (DIG)-labeled locked nucleic acid (LNA) detection probes targeted to the mature form of miR-128 (Figure 1A). As previously reported (Tan et al., 2013), miR-128 was found to be predominantly expressed in the brains and spinal cords of E14.5 mice, and no signal was detected with a scrambled miRNA probe (Figure 1A). As an alternative method, we performed quantitative real-time PCR (qPCR) and found spatial expression patterns of miR-128 that were similar to those observed using ISH (Figure 1B). Within the E14.5 forebrain, miR-128 was clearly detectable in the cortical layers, and high-magnification images of cortical slices at E14.5 revealed the expression of miR-128 in cells within the ventricular/subventricular zone (VZ/SVZ) (Figure 1C). To further confirm this, we performed fluorescence ISH in combination with immunostaining using the NPC marker NESTIN in cortical slices at E14.5 and found that NPCs within the VZ/SVZ expressed miR-128 (Figure 1—figure supplement 1). Furthermore, NPCs isolated from the E14.5 forebrain co-expressed miR-128 and NESTIN (Figure 1D), indicating the potential functional role of miR-128 in regulating the proliferation and/or differentiation of NPCs.
To examine whether miR-128 regulates the proliferation and/or differentiation of embryonic NPCs, we designed constructs for gain-of-function and loss-of-function studies. A dual-promoter expression construct carrying CMV-driven mouse miR-128-1 precursor and EF1α-driven copGFP (Hollis et al., 2009) (miR-128) was used to overexpress miR-128, while a dual-promoter expression construct containing H1-driven anti-sense miR-128 short-hairpin RNA and CMV-driven copGFP (miR-Zip-128) (Guibinga et al., 2012) was used to knock down endogenous miR-128 (Figure 2—figure supplement 1A and B). When we transfected primary embryonic NPCs that were isolated from E14.5 mouse forebrains with these constructs, the miR-128-1 precursor was efficiently processed to generate the mature miRNA, as shown by a 15-fold upregulation of miR-128 in transfected cells (Figure 2—figure supplement 1A). In addition, miR-Zip-128 transfection resulted in efficient knockdown of endogenous miR-128 (Figure 2—figure supplement 1B).
NPCs that were isolated from E14.5 mouse forebrains were electroporated with the aforementioned constructs and were pulse-labeled with 5-bromo-2’-deoxyuridine (BrdU) for 6 hr to label dividing cells of a heterogeneous group of NPCs from all phases of the cell cycle (Bez et al., 2003; Zhang et al., 2014) (Figure 2A). Overexpression of miR-128 inhibited NPC proliferation, as indicated by a 56% reduction in BrdU incorporation in transfected cells (arrowheads) compared with the incorporation in those transfected with the miRNA mimic control (Figure 2B and C). Conversely, miR-128 knockdown led to a 50% increase in the number of GFP-BrdU double-positive cells compared to the scramble control (Figure 2D and E).
To determine whether modulating the levels of miR-128 affected cell death, we performed a TUNEL assay (Cai et al., 2000) (Figure 2—figure supplement 2A–D) and antibody staining against cleaved caspase-3 (Chen and Dong, 2009) (Figure 2—figure supplement 2E–H). These results showed that upregulation or downregulation of the miR-128 level specifically affected cell proliferation without affecting apoptosis.
Next, to determine the fate of the NPCs following cell-cycle exit, NPCs were transfected with the aforementioned constructs and were subsequently induced to differentiate in vitro by withdrawing growth factors from the culture medium for 5–6 days (Ma et al., 2008; Zhang et al., 2014) (Figure 2F). Neuronal differentiation was assayed by immunostaining with TUJ1, a specific antibody against beta-III-tubulin (Ferreira and Caceres, 1992). An increase in the number of GFP and TUJ1 double-positive cells upon treatment would indicate increased neuronal differentiation, whereas a decrease would indicate the inhibition of neurogenesis. Intriguingly, miR-128 overexpression increased the neuronal differentiation of NPCs, as shown by a significant increase (of approximately 100%) in the number of GFP and TUJ1 double-positive cells (Figure 2G and H). In contrast, miR-128 knockdown resulted in a 40% decrease of GFP-TUJ1 double-positive cells compared with treatment with the scrambled control miRNA (Figure 2I and J). When we assayed neuronal differentiation using immunostaining with the marker of mature neurons MAP2, we observed a similar effect on neuronal differentiation (Figure 2—figure supplement 3A–D). Moreover, lentiviral transduction of miR-128 or miR-Zip-128, as an alternative gene delivery approach, resulted in similar effects on neurogenesis (Figure 2—figure supplement 4A–D). Taken together, these results indicate that miR-128 overexpression enhances neuronal differentiation of NPCs following cell-cycle exit, while miR-128 knockdown shows the opposite effect on neuronal differentiation.
To further test whether the enhanced differentiation was restricted to a neuronal fate, we immunostained NPCs with a specific antibody against GFAP, a marker for glial cells, and observed that miR-128 overexpression significantly decreased the number of GFP and GFAP double-positive cells, indicating that NPCs that had exited the cell cycle following miR-128 overexpression may have over-committed to neuronal differentiation, preventing NPCs from becoming glial cells. The opposite effect was observed upon miR-128 knockdown in NPCs (Figure 2—figure supplement 5A–D).
To rule out the potential bias in the in vitro cell proliferation assay we monitored the time-course of BrdU incorporation using control NPCs (Figure 2—figure supplement 6A) as well NPCs electroporated with miR-ZIP-128. We found that the effects of miR-128 knockdown in NPCs (increased cell proliferation compared to control) remained unchanged at most of the time points (Figure 2—figure supplement 6B).
During development, NPCs in the VZ maintain proliferative capacity and the ability to self-renew (apical progenitors, APs). These APs give rise to intermediate progenitors (basal progenitors, BPs) within the SVZ and IZ that can proliferate and become neurons (Martinez-Cerdeno et al., 2006; Pontious et al., 2008). To examine the role of miR-128 during neocortical development in vivo, we introduced miR-128 and miR-Zip-128 into the lateral ventricular wall of E13.5 mouse brains by in utero electroporation and analyzed the electroporated brains at E14.5.
First, to detect changes in NPC proliferation, we monitored the mitotic spindle orientation (Huttner and Kosodo, 2005; Wang et al., 2011) of APs within the VZ that were undergoing mitosis using an antibody against phosphorylated histone H3 (PH3) (Postiglione et al., 2011), which labels dividing nuclei (Figure 3A). We found a significant decrease in the percentage of horizontal divisions upon miR-128 overexpression (Figure 3A and B, 10% ± SD), while miR-128 knockdown led to a significant increase in this percentage (Figure 3A and C, 20% ± SD). Based on these observations, we performed further experiments to identify the fate of AP progeny following miR-128 overexpression and miR-128 knockdown.
Furthermore, miR-128 overexpression led to a marked decrease in the percentage of cells that were positively labeled for the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) (Ishino et al., 2014) (~12%) (Figure 3D and E), reduced cell division, as indicated by immunostaining with Ki67 (Figure 3—figure supplement 1A and B), and a marked decrease in the number of cells that were positive for the AP marker PAX6 (28% reduction in the number of VZ/SVZ cells that were positive for both GFP and PAX6) (Figure 3H and I) and SOX2 (Figure 3—figure supplement 2A and B). These data indicate that miR-128 overexpression decreased the number of proliferating APs within the VZ/SVZ. In contrast, miR-128 knockdown had the opposite effects on EdU incorporation and on Ki67, PAX6 and SOX2 immunostaining in APs (Figure 3F,G,J and K) (Figure 3—figure supplement 1C and D, 2C and D).
Next, given that we observed increased numbers of obliquely dividing cells upon miR-128 overexpression (Figure 3A and B), indicating potential expansion of BPs (Huttner and Kosodo, 2005; Wang et al., 2011), we monitored TBR2 expression upon miR-128 overexpression. MiR-128 overexpression led to an increase in the number of TBR2-positive cells (60% increase in GFP-TBR2 double-positive cells) (Figure 3L and M), while miR-128 knockdown resulted in a decrease in the number of TBR2-positive cells (30% decrease in GFP-TBR2 double-positive cells) (Figure 3N and O). Taken together, these data indicate that miR-128 regulates NPC proliferation by promoting intermediate basal progenitors at the expense of apical progenitors within the VZ/SVZ.
Because BPs will generate the bulk of cortical neurons (Tan and Shi, 2013), we tested whether miR-128 regulates the neuronal differentiation of NPCs in vivo. First, we analyzed the number of BPs that exited the cell cycle (Ge et al., 2010; Yang et al., 2012) upon miR-128 manipulation. Following in utero electroporation, dividing cells were labeled with EdU for 24 hr and subsequently immunostained for Ki67. Compared to the control treatment, miR-128 overexpression increased the number of cells that were positive for both GFP and EdU but negative for Ki67 cells (by ~40%), indicating an increase the number of BPs that had exited the cell cycle (Figure 3P and Q). Conversely, miR-128 knockdown had the opposite effect (Figure 3R and S). To further examine whether the observed changes in cell cycle exit ultimately led to a difference in neurogenesis, we analyzed brains at E17.5, four days after electroporation, and assayed neuronal differentiation by immunostaining with a specific antibody against the neuronal marker NEUN (Zhang et al., 2014). miR-128 overexpression significantly increased (by ~25%) the number of NEUN-positive neurons in the CP zone compared with the number in the control condition (Figure 3T and U). Moreover, miR-128 knockdown had the opposite effect on neurogenesis (Figure 3V and W). Taken together, these results indicate that miR-128 may act on two different stages of NPC development: first, by regulating symmetric/asymmetric division of APs, miR-128 promotes BP production; and second, by enhancing the exit of BPs from the cell cycle, miR-128 promotes overall neurogenesis (Figure 3—figure supplement 3). In contrast, downregulation of miR-128 in early neuronal precursors impeded their developmental progression by causing them to be retained in a more primitive, proliferative stage, resulting in the expansion of a pool of the NPC pool. Intriguingly, this early expansion of NPC pools upon miR-128 knockdown did not result in a net increase in neuronal numbers at E17.5, suggesting that further investigation is necessary to delineate whether the observed phenomena is due to compromised neurogenic capability of NPCs or simply due to a delay in neurogenesis which could eventually be overcome at a later postnatal stage (Figure 3—figure supplement 3).
miRNAs normally regulate the translation and/or degradation of multiple target mRNAs (Bartel, 2009). To further characterize the molecular mechanisms that underlie the changes in NPC competence, we utilized two widely used in silico microRNA target prediction algorithms (TargetScan and miRanda) (Mi et al., 2013; Witkos et al., 2011), which identified 800 and 940 potential targets of miR-128, respectively (Figure 4—source data 1). Among the 77 overlapping targets identified using two algorithms, only 53 genes were annotated to have known biological processes and thus selected for further testing (Figure 4—source data 1). qPCR analysis of cultured NPCs that overexpressed miR-128 revealed 21 genes that were downregulated (Figure 4—source data 2). Eleven out of the 53 genes tested exhibited consistent reduction in mRNA levels upon miR-128 overexpression in cultured mouse NPCs (Pcm1, Lmbr1l, Foxo4, Sh2d3c, Nfia, Pde8b, Sec24a, Pde3a, Fbxl20, Ypel3 and Kcnk10, Figure 4—source data 2, highlighted in yellow). The 11 genes were further tested for reciprocal upregulation when miR-128 was inhibited. qPCR analysis following miR-128 inhibition showed that only Pcm1, Nfia, Foxo4, and Fbxl20 were consistently upregulated among which Pcm1 displayed the greatest change (Figure 4—source data 3).
We further validated Pcm1 as a target of miR-128 using a luciferase assay. First, we cloned the 3’-UTR of Pcm1 (WT-Pcm1) into a dual-luciferase reporter construct, pmirGLO, to assess translation of the target protein based on the luciferase activity (Krishnan et al., 2013) (Figure 4A). In this assay, co-transfection of miR-128 with the WT-Pcm1 reporter construct markedly suppressed the luciferase activity (by 58%, Figure 4B). However, co-transfection of miR-128 with random 3’-UTR sequences (Control, Figure 4B) did not affect the luciferase activity. To further determine whether the targeting of PCM1 by miR-128 was specific, we introduced three mismatched nucleotides to the predicted seed region of the miR-128 binding site (MT-Pcm1) (Figure 4A, red underlines). Mutating these seed sequences abolished the miR-128-mediated suppression of PCM1 luciferase activity and restored the luciferase activity to the control level (Figure 4B), indicating the specificity of miR-128 targeting of the 3’-UTR of Pcm1.
Next, we examined whether miR-128 downregulated the endogenous expression of PCM1 at the mRNA and protein levels by overexpressing either miR-128 or a scrambled control in NPCs and then performing qPCR and western blot analyses using a specific antibody against PCM1. We observed that miR-128 overexpression significantly reduced PCM1 mRNA (Figure 4C) and protein levels (Figure 4D). Conversely, miR-128 knockdown using a specific inhibitor of miR-128 (anti-miR-128) (Smrt et al., 2010), in comparison to using a scrambled anti-miR (anti-miR-control) (Figure 4—figure supplement 2) in NPCs produced the opposite effect on the expression of PCM1 mRNA (Figure 4E) and protein (Figure 4F). Taken together, these data suggest that miR-128 targets PCM1 expression in NPCs, which in turn controls NPC proliferation and differentiation in vitro. In addition, qPCR analyses of tissue samples that were isolated from the VZ/SVZ, IZ, and CP using laser capture microdissection (LCM) (Wang et al., 2009) revealed an inverse relationship between miR-128 and PCM1 mRNA (Figure 4G and H). An inverse relationship between miR-128 and PCM1 mRNA levels was also evident temporally, given that the expression of miR-128 gradually increased starting from E12.5 through P0, whereas PCM1 protein expression was found to gradually decrease over this time period (Figure 4—figure supplement 3A–B), suggesting that miR-128 might regulate PCM1 levels to control NPC proliferation and differentiation in the developing cortex.
If the effect of miR-128 on the proliferation and differentiation of NPCs is mediated through the suppression of endogenous PCM1, then PCM1 downregulation should mimic the cellular effects of miR-128 overexpression. To test this hypothesis, we generated two small hairpin RNA (shRNA) vectors that expressed shRNAs that targeted mouse PCM1 and validated the efficiency of these shRNAs in knocking down PCM1 in mouse neuroblastoma (N2A) cells (Figure 5—figure supplement 1A). The expression of the shRNA vectors #1 and #2 led to a reduction in endogenous PCM1 of approximately 60 and 70%, respectively. Based on these data, we used shRNA #2 to examine the role of PCM1 in early neurogenesis.
PCM1 has been shown to affect the proliferation and neurogenesis of NPCs; knockdown of PCM1 inhibits NPC proliferation but promotes NPC differentiation in the developing cortex (Ge et al., 2010). To further confirm the loss of PCM1 function in NPCs, we electroporated NPCs with PCM1 shRNA and assessed the proliferation of NPCs using BrdU pulse-labeling for 6 hr. The reduction of endogenous PCM1 via shRNA led to significant inhibition of NPC proliferation, as indicated by a 35% reduction in BrdU incorporation by transfected cells compared with the BrdU incorporation by NPCs that were transfected with a control scrambled shRNA (Figure 5A and 5B); this reduction was comparable to that caused by the overexpression of miR-128 in NPCs (Figure 2B and C). PCM1 knockdown did not affect cell survival, as indicated by TUNEL assay and antibody staining against activated caspase-3 (Figure 5—figure supplement 2A–D).
Next, to determine whether knocking down endogenous PCM1 could trigger the neuronal differentiation of NPCs, NPCs were transfected with PCM1 shRNA or a scrambled control shRNA, induced to differentiate, and immunostained for TUJ1 (Figure 5C and D). Similar to miR-128 overexpression, PCM1 shRNA expression significantly increased the neuronal differentiation of NPCs (by approximately 60% compared with that of scrambled shRNA-transfected NPCs, Figure 5C and D). Consistent with this finding, MAP2 immunostaining revealed a similar increase in the neuronal differentiation of PCM1 shRNA-expressing NPCs (Figure 5—figure supplement 3A and B). Taken together, these results indicate that inhibiting PCM1, which is a target of miR-128, mimics the cellular effect of miR-128 on NPC proliferation and differentiation.
To examine whether PCM1 overexpression would exert the opposite effect of PCM1 knockdown in NPCs, we co-expressed PCM1 (without its 3’-UTR) in NPCs using a GFP expression construct and found that PCM1 overexpression increased the proliferation of NPCs compared to the vector-only control treatment (70% increase in BrdU and GFP double-positive cells) (Figure 5—figure supplement 4A and B). However, PCM1 overexpression decreased neurogenesis, as determined by immunostaining for TUJ1 (35% decrease in the number of TUJ1 and GFP double-positive cells) (Figure 5—figure supplement 5A and B) and MAP2 (45% decrease in the number of MAP2 and GFP double-positive cells) (Figure 5—figure supplement 5C and D). TUNEL and activated caspase-3 staining demonstrated that PCM1 overexpression had no effect on apoptosis (Figure 5—figure supplement 6). We confirmed these in vitro findings in vivo by electroporating PCM1 into NPCs in the VZ of E13.5 mouse brains. First, we monitored the mitotic spindle orientation of APs within the VZ that were undergoing mitosis by labeling the dividing nuclei with PH3 (Figure 5—figure supplement 7A). We observed a significant increase in the percentage of horizontally dividing cells upon PCM1 overexpression (Figure 5—figure supplement 7B, 20% ± SD). In addition, the expression of exogenous PCM1 in vivo led to a marked increase in the number of cells that incorporated EdU (40% increase in EdU and GFP double-positive cells) (Figure 5—figure supplement 7C and D). Furthermore, we observed a marked increase in the number of PAX6-positive apical progenitor cells (60% increase in PAX6 and GFP double-positive cells) (Figure 5—figure supplement 7E and F) and a decrease in the number of TBR2-positive intermediate progenitor cells in brains in which NPCs had been electroporated with PCM1 (40% decrease in TBR2 and GFP double-positive cells) (Figure 5—figure supplement 7G and H). We monitored the neuronal differentiation of PCM1-expressing NPCs in vivo using NEUN staining and observed a 25% decrease in the neuronal differentiation of the NPCs (Figure 5—figure supplement 7I and J).
To determine whether the effect of miR-128 on neuronal differentiation is mediated through PCM1, we co-electroporated cells with miR-128 and a PCM1 expression vector lacking the 3’-UTR. A vector-only construct was used as the corresponding control construct for PCM1 overexpression. We found that PCM1 overexpression rescued the decrease in NPC proliferation (103% versus 62% for BrdU and GFP double-positive cells, Figure 6A and B), and reversed the observed increase in neuronal differentiation induced by miR-128 overexpression (150% versus 225% for TUJ1 and GFP double-positive cells, Figure 6C and D; 115% versus 160% for MAP2 and GFP double-positive cells; Figure 6—figure supplement 1A and B) in vitro.
To further verify these results in vivo, we co-electroporated the PCM1 expression vector with miR-128 in utero. Similarly, we found that co-expression of PCM1 with miR-128 successfully reversed the effects of miR-128 on neural stem cell proliferation (100% versus 75% for EdU and GFP double-positive cells, Figure 7A and B), PAX6 (78% versus 60% for PAX6 and GFP double-positive cells, Figure 7C and D), and TBR2 expression (120% versus 160% for TBR2 and GFP double-positive cells, Figure 7E and F) as well as on neuronal differentiation (100% versus 123% for NEUN-positive cells, Figure 7G and H). Taken together, these results strongly suggest that miR-128 regulates the proliferation and differentiation of NPCs in the murine embryonic cortex by targeting PCM1 expression though a direct interaction with its 3’UTR.
The cerebral cortex, which is the most complex structure of the brain, is responsible for cognitive, motor and perceptual behaviors (Volvert et al., 2012). The generation of cortical neurons depends on NPCs exiting the cell cycle, migrating, and subsequently partially maturing into neurons. These processes are orchestrated by multiple gene products that ultimately converge on the cytoskeleton to support morphological remodeling (Sun and Hevner, 2014). Therefore, for all of these processes to be correctly executed, the timing and abundance of specific gene products must be precisely regulated, and a lack of regulation may result in the cortical malformations that are associated with certain neuropsychiatric disorders (Zhang et al., 2014).
miRNAs are abundant, short-lived double-stranded RNAs of ~20-25 nucleotides that are derived from endogenous short-hairpin transcripts (Bartel, 2009). miRNAs contribute to various developmental processes by acting as post-transcriptional regulators; thus, they introduce an additional level of intricacy to gene regulation in neurogenesis. Recent data obtained by several groups support a primary role of miRNAs in fine-tuning signaling pathways that control the synchronized steps of cortical development (Kawahara et al., 2012; Shi et al., 2010).
Abu-Elneel et al. investigated the expression of 466 human miRNAs from postmortem cerebellar cortical tissue from individuals with ASDs and identified twenty-eight miRNAs that were expressed at significantly different levels in the ASD brains compared with the non-autism control brains (Abu-Elneel et al., 2008). Interestingly, of these dysegulated miRNAs, only three of them (miR-7, miR-128, and miR-132) were exclusively expressed in the brain (Li and Jin, 2010).
miR-128 is transcribed from two distinct loci, miR-128-1 and miR-128-2, as two primary transcripts that are processed into identical mature miRNA sequences (Adlakha and Saini, 2014). miR-128-1 and miR-128-2 reside in the intronic regions of genes on two different chromosomes (Tan et al., 2013). Previously, downregulation of miR-128 has been reported in several brain cancers, including glioblastoma and medulloblastoma (Adlakha and Saini, 2013; Peruzzi et al., 2013). Consistent with these findings, allelic loss of chromosome 3p, where miRNA-128-2 is encoded, has also been associated with the most aggressive forms of neuroblastoma, indicating that miR-128 may play an important role in the cell cycle as well as growth and differentiation (Adlakha and Saini, 2014).
In identifying the targets of miR-128, we eventually narrowed our search of potential miR-128 targets in NPCs to Pcm1, Nfia, Foxo4, and Fbxl20 (Figure 4—source data 1). Among them, Foxo4, which encodes for an insulin/IGF-1 responsive transcription factor that regulates cell cycles (Furukawa-Hibi et al., 2005; Schmidt et al., 2002), was ruled out as a probable functional target of miR-128 based on a recent study that reported the loss of FOXO4 reduces the potential of human embryonic stem cells (hESCs) to differentiate into neural lineages (Vilchez et al., 2013), which is opposite from miR-128 overexpression effects that we observed. Nfia (Nuclear Factor I/A) encodes for a protein that functions as a transcription and replication factor for adenovirus DNA replication (Qian et al., 1995), while Fbx120, encodes for a F-box protein which is involved in synaptic plasticity of neuronal networks (Takagi et al., 2012). As such, both genes were ruled out since their known biological functions were not relevant to the neurogenesis phenotype observed in miR-128 manipulation. Based on these rationales, we sought to focus on PCM1 as our primary gene of interest and investigate the functional relationship between PCM1 and miR-128 in early neurogenesis. Furthermore, based on our expression analysis, miR-128 expression is specific to neural stem cells and gradually increases during cortical development (Figure 1). In contrast, the expression level of its target protein PCM1 is higher during early developmental stages and lower during late developmental stages (Figure 4—figure supplement 3). The inverse expression patterns of PCM1 and miR-128 indicate that miR-128 may function by turning off PCM1 expression, indicating that PCM1 may be the primary regulator by which miR-128 governs NPC proliferation and differentiation.
In this study, we sought to recapitulate the miR-128 upregulation observed in some ASD brains by overexpressing miR-128 specifically in NPCs. Importantly, we observed a dramatic change in the proliferation and differentiation of NPCs. The observed effects of miR-128 are consistent with a previous study by Ge et al. that demonstrated that the loss of PCM1 triggered NPCs to exit the cell cycle early and promoted the premature differentiation of NPCs to neurons (Ge et al., 2010). In this study, knockdown of PCM1 resulted in impaired interkinetic nuclear migration of NPCs, which leads to the overproduction of neurons and to premature depletion of the NPC pool in the developing neocortex. Consistent with this description, our results showed that miR-128 overexpression produced a phenotype similar to that previously reported for PCM1 knockdown (Figures 2 and 3). More importantly, we found that this phenotype could be rescued by the co-expression of a miR-128-resistant PCM1 variant (Figures 6 and 7). In contrast, knockdown of miR-128 resulted in increased levels of PCM1 in NPCs (Figures 4) and showed the opposite phenotype of miR-128 overexpression in terms of NPC proliferation and differentiation (Figures 2 and 3). Our results suggest that miR-128 regulates NPC proliferation and differentiation by fine-tuning endogenous PCM1 levels, which serve as a primary regulator that is required for proper neurogenesis to occur.
Intriguingly, we recently identified 9 novel (i.e., previously unreported) missense mutations in the Pcm1 gene in ASD patients (H.S.J. and S.G.R., unpublished observations), indicating that PCM1 misregulation might be a core mechanism in some ASD patients with disrupted cortical development. Other recent studies using miR-128-2 knockout mice indicate that miR-128 levels regulate the excitability of adult neurons (Tan et al., 2013). By selectively inactivating miR-128-2 in forebrain neurons using Camk2a-Cre and floxed miR-128-2, Tan et al. found that reduced miR-128 expression triggered the early onset of hyperactivity, seizures, and death (Tan et al., 2013). Based on their bioinformatics network and pathway analyses of miR-128 target genes, those authors found that miR-128 may regulate the expression of numerous ion channels and transporters as well as genes that contribute to neurotransmitter-driven neuronal excitability and motor activity (Tan et al., 2013). Because NPCs are not excitable due to a lack of active sodium channels (Li et al., 2008), it is unlikely that the cellular effects of miR-128 observed here resulted from changes in the expression of ion channels or transporters. However, it will be interesting to follow neurons derived from NPCs with misregulated miR-128 to characterize how these neurons integrate into and function in cortical circuits. Moreover, it will be interesting to generate miR-128-1 and miR-128-2 double knockout mice and inducible miR-128-overexpressing transgenic mice to monitor the proliferation and differentiation of NPCs and their effects on behavior.
Taken together, our results suggest that miR-128 is an important regulator of cortical development through PCM1. Future studies to further elucidate specific aspects of the roles of miR-128 and PCM1 in neuronal development and function will be of great interest to this field.
All studies were conducted with protocols that were approved by the Institutional Animal Care and Use Committee (IACUC, protocol number: 2013/SHS/809) of the Duke-NUS Graduate Medical School and National Neuroscience Institute. Time-mated C57BL/6 mice were purchased (InVivos, Singapore) at E13.5 and E14.5 for in utero electroporation and culturing of NPCs.
Mouse embryos were harvested at E14.5, and the dorsolateral forebrain was dissected and enzymatically triturated to isolate a population of cells enriched in NPCs as previously described. NPCs isolated from a single brain were suspension-cultured in a T25 tissue culture flask in proliferation medium containing human EGF (10 ng ml-1), human FGF2 (20 ng ml-1) (Invitrogen, Carlsbad, CA), N2 supplement (1%) (GIBCO), penicillin (100 U ml-1), streptomycin (100 mg ml-1), and L-glutamine (2 mM) for 5 days and were allowed to proliferate to form neurospheres.
DIV 5 neurospheres were dissociated into single cells using accutase, yielding 4–6 × 106 cells per T25 flask. For each electroporation reaction, 1 × 106 cells were mixed with 2 µg DNA and electroporated using the Neon electroporator (Invitrogen) according to the manufacturer’s protocol. Immediately following electroporation, cells were suspension-cultured in a 6-well tissue culture plate in proliferation medium and were allowed to re-form neurospheres for 24 hr.
Twenty-four hours post-electroporation, the cells were pulsed with 1 mM 5-bromo-2’-deoxyuridine (BrdU, Roche) for 6 hr. The neurospheres were then gently dissociated by pipetting and seeded onto 60 mm coverslips coated with poly-L-lysine and laminin, at a density of 2 × 104 cells/coverslip. After waiting 30 min to allow the cells to attach, the cells were fixed with 4% paraformaldehyde for 30 min at room temperature. To detect BrdU, NPCs were pre-treated with 2 M HCl for 15 min at 37°C, washed with borate buffer (pH 8.5) for 30 min and immunostained using a mouse antibody against BrdU (#8039, Abcam).
Twenty-four hours post-electroporation, neurospheres were gently dissociated by pipetting and seeded onto 60 mm coverslips coated with poly-L-lysine and laminin, at a density of 2 × 104 cells/coverslip. Subsequently, NPCs were cultured as monolayer in differentiation medium containing N2 (1%) in DMEM/F12 and were maintained for 5–6 days.
The primary antibodies included the following: rabbit anti-Ki67 (#15580, Abcam), mouse anti-beta III tubulin (TUJ1, #1637, Millipore), mouse anti-GFAP(#N206A/8, NeuroMab), mouse anti-NEUN (#MAB377, Millipore), chicken anti-MAP2 (#5392, Abcam), rabbit anti-PH3 (#9701, Cell Signaling), rabbit anti-TBR2 (#23345, Abcam), rabbit anti-cleaved caspase-3 (#9661, Cell Signaling), and rabbit anti-PAX6 (#PRB-278P, Covance). The secondary fluorochrome-conjugated antibodies were diluted 1:400 (donkey anti-mouse, donkey anti-rabbit, goat anti-mouse and goat anti-rabbit, goat anti-chicken (Invitrogen]). Nuclear counterstaining was performed with 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, #B2261at 0.25 μg/μl). TUNEL staining was carried out using a kit purchased from Roche (#12156792910). Images were obtained using an LSM710 confocal microscope (Zeiss).
For LCM, unfixed, fresh E14.5 brains were embedded in Tissue-Tek O.C.T. compound (Sakura) at -24°C. Ten-micrometer cryosections were mounted on PEN Membrane slides (Leica Microsystems, Germany) and stored at -80°C until ready for dissection. LCM of the VZ/SVZ, IZ, and CP was performed under direct visualization of the unstained tissue based on tissue morphology using an inverted microscope and PALM Robo software (Carl Zeiss, Germany). The isolated tissue samples were attached onto an Adhesive Cap-500 tube (Carl Zeiss) for direct lysis and total RNA extraction.
Lentiviral miR-128 expression plasmid and miR-128-Zip knockdown plasmid was obtained from System Biosciences. miR-128 inhibitor was obtained from Genepharma. The PCM1 expression construct was a kind gift from A. Kamiya (Johns Hopkins, US).
To generate luciferase reporter constructs, the 3’-UTR of Pcm1 was produced by PCR using murine genomic DNA library. Following primers were used: Pcm1 (1865bp), forward primer: 5’-GAACCTGAAACAGTGGGAGC-3’; reverse primer: 5’-ACGGTTGCATGTTCCCAATC-3’. The resulting PCR products were cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega).
To generate the pmirGLO-PCM1 3’-UTR mutant construct, miR-128 targeting sequences (CACTGTG) were mutated to (GAGTCTG) by the Quick Change Site-Directed Mutagenesis Kit (Stratagene) using following primers: forward primer: 5'-CCTGGACAGATTCAAACCTTGACAGAGTCTGGGATTTTTCTTTTGC-3' and reverse primer: 5'-GCAAAAGAAAAATCCCAGACTCTGTCAAGGTTTGAATCTGTCCAGG-3'.
We generated two Pcm1 shRNA vectors with two different published targeting sequences (Kamiya et al., 2008) #1: 5’-AGCTACTTAATACAGACTA-3’ and #2: TCAGCTTCGTGATTCTA using pCDH-U6-nucGFP-Puro vector which was modified from the lentiviral miR-128 expression plasmid, MMIR-128-1-PA-1.
HEK293 cells were transfected with either the miR-128 mimic or control miRNA in conjunction with the luciferase reporter constructs. Forty-eight hours after they were transfected, the cells were lysed and subjected to luciferase assays using the Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol.
Total RNA was extracted using the miRNeasy kit (Qiagen) from tissue samples or NPCs. The extraction procedure was then followed by cDNA synthesis using a cDNA synthesis kit (Promega). PCRs were performed on three independent sets of template, and the cycling parameters were as follows: 94°C for 15 s, 55°C for 30 s, and 70°C for 30 s for 40 cycles using the CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA). For each assay, PCR was performed after a melting curve analysis. To reduce variability, we ran each sample in duplicate or even triplicate and included control qPCR reactions without template for each run. The qPCR primers are listed in Figure 4—source data 4.
Timed-pregnant mice (E13.5) were anesthetized with isoflurane (induction, 3.5%; surgery, 2.5%), and the uterine horns were exposed by laparotomy. The DNA (3–5 µg µl-1 in water) together with the dye Fast Green (2 mg ml-1; Sigma Aldrich, St. Louis, MO) was injected through the uterine wall into one of the lateral ventricles of each embryo using a 30-gauge Hamilton syringe. Approximately 2 μl of DNA and dye solution was delivered using a pressure injector (Picospritzer III; General Valve, Pine Brook, NJ). For electroporation, five electrical pulses (amplitude, 35 V; duration, 50 ms; intervals, 950 ms) were delivered with a BTX square-wave electroporation generator (Harvard Apparatus, Holiston, MA). The uterine horns were placed back into the abdominal cavity after electroporation, and the embryos were allowed to continue their normal development. Electroporated mouse brains were harvested at E14.5 and E17.5 for the proliferation and differentiation analyses, respectively.
The mitotic spindle orientation of dividing cells was determined on micrographs by measuring the angle of chromosomes relative to the ventricular surface. Nuclei were counterstained with DAPI, and mitotic cells were immunostained with PH3. GFP-PH3 double-positive cells within the VZ were identified on micrographs, and approximately 150 cells per experimental condition were used for quantification.
For proliferation assays, E14.5 pregnant dams were administered 5-ethynyl-2’-deoxyuridine (5 mg per kg body weight dissolved in 0.9% saline) (EdU, ThermoFisher Scientific) by intraperitoneal injection, and the embryos were harvested 2 hr later. For cell cycle exit assays, E13.5 pregnant dams were IP injected with 5 mg per kg body weight EdU immediately following in utero electroporation, and the embryos were harvested 24 hr later. Visualization of EdU was performed in accordance with the manufacturer’s protocol.
A 5’- digoxigenin (DIG)-labeled locked nucleic acid (LNA) miR-128 ISH detection probe (Exiqon) was used to detect miR-128 expression in the brain. The sequence of the probe was 5’-AAAGAGACCGGTTCACTGTGA-3’. Briefly, E12.5 to P0 perfused brains were dehydrated in 30% sucrose, embedded with Tissue-Tek, and sectioned at 12 µm (coronal sections) or 14 µm (sagittal sections). Next, the brain slices were treated with 10 μg ml-1 Proteinase K at 37°C for 15 min, followed by incubation with the LNA-DIG-labeled miR-128 detection probes (50 nM) at 50°C overnight. The sections were blocked with PBS containing 0.1% Triton X-100, 10% normal goat serum (NGS), and 0.2% bovine serum albumin (BSA) and were incubated with an AP-conjugated anti-DIG antibody (1:1000) in PBS at 4°C overnight. The NBT+CIP substrate was then added (#K2191020, BioChain Institute, Newark, CA). For simultaneous miR-128 ISH and immunostaining, the brain slices were incubated with primary antibodies (peroxidase-conjugated anti-DIG, 1:200 [Roche]; mouse anti-NESTIN, 1:1000 [Sigma]) in Tris-Buffered Saline (TBS) containing 0.1% Tween-20, 20% NGS and 0.1% BSA. Immunostaining was detected using Alexa 488, Alexa 555 or Alexa 647 fluorescent secondary antibodies. Slices were mounted with Vectashield containing DAPI (Vector Labs, Burlingame, CA) and examined with confocal microscopy.
For the analysis of electroporated brain areas, volumetric z-stacks were acquired for each experimental condition using an LSM710 laser confocal microscope and the Z-series images were collapsed with a maximum intensity projection to a 2D representation. To quantify NPC neurogenesis, the Z-series images were taken using a 40x oil immersion objective (NA 1.3) in the CP zone. To quantify the proliferation of NPCs, the Z-series images were taken using a 60x oil immersion objective (NA 1.3) in the SVZ. Approximately 1500–2000 GFP-positive cells were counted for each condition, and at least three sets of independent experiments were performed and manually quantified in a blind manner.
Cells or brain tissues were lysed using RIPA buffer containing phosphatase and protease inhibitors. Proteins were separated by SDS–PAGE under reducing conditions and transferred to polyvinyl difluoride (PVDF) membranes (Millipore, Billerica, MA). Antibody incubations were performed in 5% BSA in TBS buffer using following antibodies: anti-PCM1, 1:1000(Cell Signaling, Danvers, MA); anti-β-actin, 1:1000 (Santa Cruz, Dallas, TX); and HRP-conjugated rabbit or mouse antibodies, 1:3,000 (GE Life Science, Pittsburgh, PA).
Recombinant lentiviral particles were produced by co-transfection of HEK293T cells with 6 μg of pMD2G-VSVG, 6 μg of PAX2 packaging plasmids, and 0 μg of pCDH-U6-shRNA-nucGFP-Puro plasmid construct using Lipofectamine 2000 (Life Technologies). Lentiviral particles were collected from supernatant after 72 hr by using PEG-it kit (System Biosciences). Lentiviral particles were concentrated up to 2 × 105 TU/μL.
At least 3 experiments were performed independently under each experiment condition, and similar results were obtained. Statistical analyses were performed using ANOVA and Student’s t-test. All data were presented as mean and standard deviation (mean ± SD). Statistical significance was defined when ***p<0.001, **p<0.01, *p<0.05 compared with the control.
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Eunjoon KimReviewing Editor; Korea Advanced Institute of Science and Technology, Republic of Korea
In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.
[Editors’ note: a previous version of this study was rejected after peer review, but the authors submitted for reconsideration. The first decision letter after peer review is shown below.]
Thank you for choosing to send your work entitled "miR-128 Regulates Proliferation and Neurogenesis of Neural Precursors by Targeting PCM1 in the Developing Neocortex" for consideration at eLife. Your full submission has been evaluated by a Senior editor, a Reviewing editor and three peer reviewers, and the decision was reached after discussions between the reviewers. Based on our discussions and the individual reviews below, we regret to inform you that your work will not be considered further for publication in eLife.
1) The conclusion of the first paragraph is: "qPCR confirmed the observed neural tissue-specific miR-128 expression." But none of the preceding data suggests specificity. For example, "miR-128 was expressed at low levels in the subventricular zone (SVZ) and in the cortical plate (CP) at E14.5…" and "…we performed quantitative real-time PCR (qPCR) and found a similar temporal enrichment of miR-128 in the forebrain, which contains both the SVZ and the CP". Also, "…miR-128 was found to be predominantly expressed in the brain and spinal cord of E14.5 mice…". This does not strike the reviewer as specificity.
2) The authors claim: "High-magnification images of cortical slices at E14.5 revealed the expression of miR-128 in cells within the SVZ and the CP but not within the intermediate zone (IZ) (Figure 1C)." The enrichment of the labeling looks convincing for the CP but there is no obvious distinction between the SVZ and the IZ.
3) The isolation procedure of NPCs requires more details regarding its efficiency.
4) The authors state: "The percentage of BrdU- positive but Ki67-negative cells was increased by 350% in NPCs overexpressing miR-128 (Figure 2C, D, arrowheads for BrdU-positive, Ki67-negative cells), indicating that the ectopic expression of miR-128 stimulated NPCs to exit the cell cycle." The conclusion regarding exit from the cell cycle does not follow from the BrdU -positive/Ki67 negative cells.
5) TUNEL is a relatively insensitive way to detect apoptosis and should be complemented by other more conclusive methods.
6) What was the efficiency of the transfection with CMV-promoter-driven expression of the mouse miR-128-1 precursor and EF1α promoter-driven copGFP constructs?
7) The claim of an effect on neuronal differentiation is a good start, but their study requires more detail regarding exactly what the effects are on differentiation and on specific cell types. The paper as it now stands reads like an isolated observation without larger conceptual insights arising from the data presented.
The development of the cerebral cortex involves a tight coordination of progenitor proliferation, neuron migration and differentiation. The present work analyses the function of miR-128 during cerebral cortex development, which is a microRNA misregulated in autism spectrum disorder. The findings suggest that miR-128 is expressed by cortical progenitors where it controls their proliferation and differentiation by repressing the expression of PCM1, a protein previously described as critical for cell-cycle regulation. Ectopic expression of miR-128 promotes cell cycle exit and neurogenesis in vitro as well as in the cortex in vivo. This phenotype is rescued by PCM1 gain of function and mimicked by its loss expression, suggesting that PCM1 is a downstream target of miR-128 critical for cortical neurogenesis. Thus miR128 may belong to the machinery that coordinate cell cycle exit with neuronal differentiation.
1) According to its marked detection in the CP but not in VZ/SVZ, miR-128 has recently been shown to be solely important for late aspects of corticogenesis (migration and differentiation of projection neurons) (Franzoni et al., 2015). The results of the present findings suggest an early function of miR-128 and the reviewer highly recommends clarification of the discrepancy observed between both works.
2) The authors claim that miR-128 is not expressed in the IZ. The low magnification in Figure 1C is not conclusive and doesn't allow the reviewer to take a conclusion. One suitable experiment is qRT-PCR analysis of miR-128 and its corresponding pre-miR128 on laser-captured cortical wall regions.
3) The described gain of function phenotypes in vitro (Figure 2) is incomplete and should include more information about the experimental procedures in the text (e.g. length of BrdU pulses for cell cycle exit, timing of cultures, etc.).
4) Additional loss of function experiments (using antagomiRs or sponges against endogenous miR-128) should be done to support a physiological function of miR-128 in NPCs. This remark also stands for in utero (in vivo) experiments.
5) The monitoring of AP (=RGC) and IP cell populations using Pax6 and Tbr2, respectively is inconclusive. Other markers should be checked (such as Sox2 for AP and Insm1 for IP) to exclude specific marker loss after miR-128 expression rather than progenitor population loss.
6) The authors claim that overexpression of miR-128 "accelerates" cell cycle exit in vivo, however this is not experimentally demonstrated. In addition, promoting the generation of IPs is not really matching with increase cell cycle exit as these progenitors retain the ability to cycle and generate the bulk of projection neurons. This should be clarified and rephrased in the text.
7) It is not clear what parameter overexpression of miR-128 is affecting in APs. Is it only promoting the generation of IP or also inducing direct neurogenesis? This should be experimentally addressed. Moreover, these experiments should be complemented by loss of function studies as mentioned above. In its present form, the manuscript does not address any physiological roles of mir-128 in corticogenesis.
8) While there is no doubt that miR-128 can target PCM1 mRNAs in vitro, the expression of PCM and its targeting by endogenous miR-128 should be assessed in vivo (immunolabelings, etc.). Along this line the results obtained with functional experiments performed in vitro with miR-128 or PCM1 (proliferation and differentiation) should be confirmed in vivo. This is important because cultured NPC are different from resident APs and IPs (gene expression pattern deregulated to some extent).
In this manuscript, the authors investigated the function of miR-128 in cortical neural differentiation. They showed that miR-128 enhances neuronal differentiation and represses proliferation of NPCs through PCM1. The results are interesting. However the data are limited and impact is moderate. I have several major concerns.
First, the experiments are not complete. For gene or miRNA functional assays, both gain and loss of function assays should be performed, but not. The justification of ASD link is based on a previous study showing that miR-128 is one of 28 microRNAs dysregulated in at least one ASD individuals (for miR-128, the level is decreased in one ASD) (Abu-Elneel Neurogenetics 2008). However, several experiments only show the impact of overexpression of miR-128, not reduction, on NPCs.
Second, the in vitro NPC assays were done using electroporation which introduces a large amount of genetic materials into the cells. Electroporation also leads to a lot of cell death which is well known. However, these are not addressed. These experiments should be done, or at least confirmed using lentiviral infection.
Third, the paper is not well prepared. There is a lack of details both in methods and in legends.
Fourth, many of the experiments do not have appropriate controls.
Fifth, the statistical analyses were not done correctly.
[Editors’ note: what now follows is the decision letter after the authors submitted for further consideration.]
Thank you for resubmitting your work entitled "MiRNA-128 Regulates the Proliferation and Neurogenesis of Neural Precursors by Targeting PCM1 in the Developing cortex" for further consideration at eLife. Your revised article has been favorably evaluated by a Senior editor, a Reviewing editor, and three reviewers. The manuscript has been improved but there are some remaining issues that need to be addressed before acceptance, as outlined below:
Briefly, the reviewers pointed out some misinterpretations, unclear descriptions, and technical shortcomings, and suggested to perform some more experiments to clarify these issues. More importantly, the reviewers raised a concern about the lack of the data from knockout mice, although I am well aware of that you already have in utero electroporation loss-of-function data (semi-in vivo data) in the manuscript. These data may take a while to obtain, but you may already be close to these data or can consider using the Crispr technology to speed up the processes. Inclusion of these data, in addition to the above mentioned revision experiments, would much strengthen the manuscript.
In this revised application, the authors have provided a substantial amount of data. In fact, the authors have provided new data to address all of my concerns, except for one, which is evidence for in vivo targeting of PCM1 by miR-128. However, given the substantial other evidence supporting the regulation of PCM1 by miR-128, I consider this is an optional data at this point. I suggest acceptance for publication.
1) They note that an early expansion of NPC pools by miR-128 knockdown did not result in a net increase in neuronal numbers. That is a curious result and their explanation regarding a critical time window does not seem like an adequate explanation.
2) They did a number of experiments to validate PCM1 as a target miR-128, but they failed to do one of the most important experiments, i.e. show that the "anti-miR" can increase the endogenous protein. They show that miR-128 over expression can reduce the protein, but over-expression experiments are never as strong as the knock down experiment.
3) They need to make clearer how they chose genes for qPCR. Did they look at every gene in their list of predicted targets? Which list? How was the list filtered? How did they settle on PCM1 among all the genes affected by over-expression? Was it the only one also affected by the miR-128 inhibitor? What is the role of the other genes on the list that are potential miR-128 targets?
4) Did PCM1 knock down or over-expression affect spindle orientation?
5) miRNAs generally have small effects-they tend to buffer cells to assure a precision outcome. This paper would lead one to think that miR-128 has effects in development that go far beyond what is likely to be the in vivo role of this miRNA. The system they are analyzing which controls neural precursor numbers and spindle orientation in precursors is likely to be far more complex than what is presented here, and hence the paper seems like a simplification of a much deeper biological question.
The updated version of the manuscript includes new set of data that answer several of my concerns. However, I have additional comments about the manuscript in its present form.
1) One major issue concerns the interpretation of the data obtained in vitro with BrdU incubation. According to their clarified BrdU experiment procedure. I noticed a misinterpretation of the data. Indeed, conversely to its fast clearance from brain tissue in vivo, the BrdU remains pretty stable (for several hours) in the culture medium and it is necessary to replace it after a short time to properly assess a single cohort of cells undergoing S-phase. This is an issue when it comes to monitor cell cycle exit rate of such cohort (Figures 2G–2J; 5C). In these bioassays, the cell cycle rate exit is totally biased by the continuous labelling (during 24 hours or so) of proliferative cells undergoing S-phase (which is indeed different between conditions). Same comments stand when assessing S-phase in proliferation assays with 6hours of incubation in BrdU-containing medium.
2) The conclusion about mitotic spindle orientation (lanes 169-17) is incorrect. The Figures 3A-3C indeed show that acute modulation of miR128 expression affect spindle orientation. It is not possible to conclude that default of mitotic spindle orientation leads to impaired APs division (symmetric vs asymmetric). This has been discussed in several recent publications. A clonal analysis would be required to follow the fate of the AP progenies after miR-128 modulation. One complementary set of experiments to decipher the functional impact of mitotic spindle orientation modification would be to focally electroporate APs (with miR-128 or miR-Zip-128) and identify the fate of the progenies 24 hours later (Tbr1 for neuron, Pax6 for APs and Tbr2 for IPs).
[Editors' note: further revisions were requested prior to acceptance, as described below.]
Thank you for submitting your work entitled "MiRNA-128 Regulates the Proliferation and Neurogenesis of Neural Precursors by Targeting PCM1 in the Developing cortex" for consideration by eLife. Your article has been reviewed by two peer reviewers, and the evaluation has been overseen by a Reviewing Editor and a Senior Editor.
The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.
The current version of the manuscript with additional data has significantly improved, compared with the previous version.
However, there is still one remaining point that should be fixed. Like mentioned previously, keeping BrdU in NPC culture (which is more stable than in vivo) to assess proliferation is OK if the authors do not refer to a single cohort of cells that have undergone S-phase (otherwise the authors should have only treated cells for about an hour with BrdU).
Now, concerning the cell cycle exit rate, we usually monitor a single cohort of cell that has exited the cell cycle. It is thus not possible to measure it properly if the BrdU is kept during 24 hours in vitro (stable) because several cohorts of cells will undergo S-phase at different timing, further leading to biased analyses. However, the cell cycle exit is properly addressed in vivo (where clearance of BrdU is fast). I would thus suggest to get rid of the in vitro data that are poorly executed.https://doi.org/10.7554/eLife.11324.038
- Wei Zhang
- Hidayat Lokman
- Hyunsoo Shawn Je
- Li Zeng
- Hyunsoo Shawn Je
- Eng King Tan
- Li Zeng
- Eng King Tan
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
We are grateful to Dr. Atsushi Kamiya (Johns Hopkins) for mouse PCM1 expression vectors. Also, we thank Drs. Jaewon Ko and Shirish Shenolikar for their critical comments on the manuscript and helpful advice. This work was supported by Singapore Translational Research (STaR) Investigator Award to EKT, A*STAR BMRC TCRP Grant (13/1/96/19/688, to HSJ and LZ), Duke-NUS Signature Research Program Block Grant (to HSJ), and NNI Research Grant (to LZ).
Animal experimentation: All studies were conducted with protocols that were approved by the Institutional Animal Care and Use Committee (IACUC, protocol number: 2013/SHS/809) of the Duke-NUS Graduate Medical School and National Neuroscience Institute.
- Eunjoon Kim, Reviewing Editor, Korea Advanced Institute of Science and Technology, Republic of Korea
- Received: September 2, 2015
- Accepted: January 22, 2016
- Version of Record published: February 17, 2016 (version 1)
© 2016, Zhang et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.