Molecular mechanism of thermosensory function of human heat shock transcription factor Hsf1

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Cite this article as: eLife 2016;5:e11576 doi: 10.7554/eLife.11576

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Abstract

The heat shock response is a universal homeostatic cell autonomous reaction of organisms to cope with adverse environmental conditions. In mammalian cells this response is mediated by the heat shock transcription factor Hsf1, which is monomeric in unstressed cells and upon activation trimerizes, and binds to promoters of heat shock genes. To understand the basic principle of Hsf1 activation we analyzed temperature-induced alterations in the conformational dynamics of Hsf1 by hydrogen exchange mass spectrometry. We found a temperature-dependent unfolding of Hsf1 in the regulatory region happening concomitant to tighter packing in the trimerization region. The transition to the active DNA binding-competent state occurred highly cooperative and was concentration dependent. Surprisingly, Hsp90, known to inhibit Hsf1 activation, lowered the midpoint temperature of trimerization and reduced cooperativity of the process thus widening the response window. Based on our data we propose a kinetic model of Hsf1 trimerization.

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Nikolai Hentze

NBE Analytical R&D, AbbVie Deutschland GmbH &Co. KG, Ludwigshafen, Germany

Competing interests
The authors declare that no competing interests exist.
Laura Le Breton

Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany

Competing interests
The authors declare that no competing interests exist.
Jan Wiesner

Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany

Competing interests
The authors declare that no competing interests exist.
Georg Kempf

Biochemiezentrum, Universität Heidelberg, Heidelberg, Germany

Competing interests
The authors declare that no competing interests exist.
Matthias P Mayer

Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany

For correspondence
M.Mayer@zmbh.uni-heidelberg.de

Competing interests
The authors declare that no competing interests exist.

Reviewing Editor

David Ron, University of Cambridge, United Kingdom

Publication history

Received: September 13, 2015
Accepted: January 18, 2016
Accepted Manuscript published: January 19, 2016 (version 1)
Version of Record published: February 22, 2016 (version 2)

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.