Role of competition between polarity sites in establishing a unique front
- Duke University School of Medicine, United States
- University of North Carolina at Chapel Hill, United States
- University of Liverpool, United Kingdom
Abstract
Polarity establishment in many cells is thought to occur via positive feedback that reinforces even tiny asymmetries in polarity protein distribution. Cdc42 and related GTPases are activated and accumulate in a patch of the cortex that defines the front of the cell. Positive feedback enables spontaneous polarization triggered by stochastic fluctuations, but as such fluctuations can occur at multiple locations, how do cells ensure that they make only one front? In polarizing cells of the model yeast Saccharomyces cerevisiae, positive feedback can trigger growth of several Cdc42 clusters at the same time, but this multi-cluster stage rapidly evolves to a single-cluster state, which then promotes bud emergence. By manipulating polarity protein dynamics, we show that resolution of multi-cluster intermediates occurs through a greedy competition between clusters to recruit and retain polarity proteins from a shared intracellular pool.
Article and author information
Author details
Reviewing Editor
- Mohan Balasubramanian, University of Warwick, United Kingdom
Version history
- Received: September 15, 2015
- Accepted: November 1, 2015
- Accepted Manuscript published: November 2, 2015 (version 1)
- Version of Record published: January 12, 2016 (version 2)
Copyright
© 2015, Wu et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Role of competition between polarity sites in establishing a unique front
eLife 4:e11611.
https://doi.org/10.7554/eLife.11611
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