Translational control of nociception via 4E-binding protein 1
- McGill University, Canada
- Institut universitaire en santé mentale de Québec, Canada
- University of Edinburgh, United Kingdom
- National Center for Complementary and Alternative Medicine, National Institutes of Health, Porter Neuroscience Research Center, United States
- Université Laval, Canada
Figures
Figure 1 with 3 supplements
Eif4ebp1-/- mice exhibit mechanical hypersensitivity and increased formalin response.
Mechanical pain sensitivity is increased in Eif4ebp1-/- mice as evident by decreased von Frey thresholds (A, n=8/genotype) and shortened latencies to attack tail clip (A, n=8/genotype), whereas …
Figure 1—figure supplement 1
Eif4ebp1/2 DKO mice, but not Eif4ebp2-/-, mice exhibit mechanical hypersensitivity and enhanced formalin responding.
Mechanical and thermal pain sensitivities are not altered in Eif4ebp2-/- mice as evident by data from von Frey and tail clip assays (A, n=9/genotype), and radiant heat paw-withdrawal and hot-plate …
Figure 1—figure supplement 2
No gross alterations in the dorsal horn of the Eif4ebp1-/- mice.
To assess the possibility of spinal cord reorganization in Eif4ebp1-/- mice, we performed immunostaining of NeuN (A), Substance P (SP, B), calcitonin gene-related peptide (CGRP, C), isolectin B4 …
Figure 1—figure supplement 3
Distribution of lentivirus-driven eGFP expression.
Lentiviruses expressing eGFP were microinjected (intraparenchymal injection (IPI)) directly into the dorsal horn of the lumbar spinal cord. Seven days later the spinal cord was extracted and …
Figure 2
Increased levels of eIF4F complex in Eif4ebp1-/- mice cause mechanical hypersensitivity and increased formalin response.
(A) Left, Immunoblot analysis of cap column pull-down proteins prepared from the spinal cord of Eif4ebp1-/- and wild-type mice (WT) treated with 4EGI-1 (10 μg, i.t. once a day for 3 days) or …
Figure 3
Excitatory and inhibitory synaptic transmissions are increased in the spinal cord of Eif4ebp1-/- mice.
Representative traces (top) of mEPSCs (A) and mIPSCs (B) from lamina II neurons in acute lumbar spinal cord slices from Eif4ebp1-/- and wild-type mice (WT). Bar graphs (bottom) show mEPSC (A) and …
Figure 4
Enhanced expression of neuroligin 1 contributes to mechanical hypersensitivity of Eif4ebp1-/- mice.
(A) General translation is not altered in Eif4ebp1-/- mice as assessed by puromycin incorporation (n=3/genotype). (B) Protein expression of neuroligin (NLGN) 1, 2 and 3 is increased in synaptosomes …
Figure 5 with 1 supplement
Reduction of neuroligin 1 levels in Eif4ebp1-/- mice normalizes the enhanced excitatory synaptic transmission. mEPSCs
(A) and mIPSCs (B) were recorded from lamina II spinal neurons of wild-type (WT), Eif4ebp1-/-, Eif4ebp1-/-/Nlgn1+/- and Nlgn1+/- mice. Top: representative traces of recordings from the four …
Figure 5—figure supplement 1
Input resistance and membrane capacitance are not altered in lamina II spinal neurons from Eif4ebp1-/-, Eif4ebp1-/-/Nlgn1+/- and Nlgn1+/- mice.
Input resistance (A) and membrane capacitance (B) of lamina II spinal neurons from wild-type (WT) (n=11), Eif4ebp1-/- (n=9), Eif4ebp1-/-/Nlgn1+/- (n=10) and Nlgn1+/- mice (n=7). No differences were …
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