Mapping oxygen concentration in the awake mouse brain

  1. Declan G Lyons
  2. Alexandre Parpaleix
  3. Morgane Roche
  4. Serge Charpak  Is a corresponding author
  1. Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, France

Abstract

Although critical for brain function, the physiological values of cerebral oxygen concentration have remained elusive because high-resolution measurements have only been performed during anesthesia, which affects two major parameters modulating tissue oxygenation: neuronal activity and blood flow. Using measurements of capillary erythrocyte-associated transients, fluctuations of oxygen partial pressure (Po2) associated with individual erythrocytes, to infer Po2 in the nearby neuropil, we report the first non-invasive micron-scale mapping of cerebral Po2 in awake, resting mice. Interstitial Po2 has similar values in the olfactory bulb glomerular layer and the somatosensory cortex, whereas there are large capillary hematocrit and erythrocyte flux differences. Awake tissue Po2 is about half that under isoflurane anesthesia, and within the cortex, vascular and interstitial Po2 values display layer-specific differences which dramatically contrast with those recorded under anesthesia. Our findings emphasize the importance of measuring energy parameters non-invasively in physiological conditions to precisely quantify and model brain metabolism.

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Author details

  1. Declan G Lyons

    Laboratory of Neurophysiology and New Microscopies,, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  2. Alexandre Parpaleix

    Laboratory of Neurophysiology and New Microscopies,, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  3. Morgane Roche

    Laboratory of Neurophysiology and New Microscopies,, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  4. Serge Charpak

    Laboratory of Neurophysiology and New Microscopies,, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France
    For correspondence
    serge.charpak@parisdescartes.fr
    Competing interests
    The authors declare that no competing interests exist.

Ethics

Animal experimentation: All animal care and experimentation was performed in accordance with the INSERM Animal Care and Use Committee guidelines (protocole number CEEA34.SC.122.12 and CEEA34.SC.123.12)

Copyright

© 2016, Lyons et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Declan G Lyons
  2. Alexandre Parpaleix
  3. Morgane Roche
  4. Serge Charpak
(2016)
Mapping oxygen concentration in the awake mouse brain
eLife 5:e12024.
https://doi.org/10.7554/eLife.12024

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https://doi.org/10.7554/eLife.12024