Mechanical sensitivity of Piezo1 ion channels can be tuned by cellular membrane tension
- Duke University Medical Center, United States
Figures
Figure 1
Electrophysiology and high-contrast imaging of Piezo-containing membranes.
Pressure-step protocol, representative currents and corresponding images from individual cell-attached patches from a HEK293t cell expressing mouse Piezo1-IRES-GFP, upon negative (A) and positive (B)…
Figure 2
Mean Piezo1 current responses for all patch configurations upon positive and negative pressure stimulation.
(A) Pressure-evoked currents from cell-attached patches from HEK293t cells expressing empty vector (pcDNA; open circles) or Mouse Piezo1-IRES-GFP (closed circles). Separate patches were tested for …
Figure 3
Measurement of membrane curvature and quantification of membrane tension.
(A) Representative image of cell-attached patch and schematic showing orientation of membrane. The solid red line marks the measured position of the membrane and the dashed yellow line is a circular …
Figure 4
Activation of Piezo1 currents upon release of pressure stimulation.
(A) Left, pressure stimulus protocol and representative currents showing activation of Piezo1 ion channels in a cell-attached patch upon release of a 5 s positive pressure stimulus. Holding …
Figure 5
Overall Piezo1 sensitivity is regulated by resting membrane tension.
(A) Stimulus protocol and representative currents from a cell-attached HEK293t cell patch expressing mouse Piezo1-IRES-GFP. The test pulse for these currents was −10 mmHg (thick purple line); …
Videos
Video 1
Response of cell-attached HEK293t patch to stimulation with negative pressure in −5 mmHg increments; acquired at 7.9 frames/s, played at 50 frames/s.
Video corresponds to cell in Figure 1A.
