1. Neuroscience
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Transcriptomics: Resting easy with a sleep regulator

  1. William J Giardino
  2. Luis de Lecea  Is a corresponding author
  1. Stanford University, United States
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Cite this article as: eLife 2015;4:e12093 doi: 10.7554/eLife.12093
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The kcnh4a gene is enriched in Hcrt neurons and promotes sleep in zebrafish.

(A) Fluorescent-assisted cell sorting (FACS) of neurons from hcrt-eGFP zebrafish followed by all-mRNA sequencing (RNA-seq) identified 212 genes that are significantly enriched in Hcrt neurons, including the voltage-gated potassium channel kcnh4a. (B) In situ hybridization for knch4a confirmed its expression in 100% of hcrt-eGFP neurons. (C) Genomic analyses revealed that kcnh4a lies directly upstream of the hcrt gene, a phenomenon that is conserved from zebrafish to humans. (D) CRISPR/Cas9 genome editing produced a mutant zebrafish with a 60% reduction in kcnh4a levels, resulting in hyperactivity and disrupted patterns of nighttime sleep.

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