1. Computational and Systems Biology
  2. Developmental Biology
Download icon

Unipolar distributions of junctional Myosin II identify cell stripe boundaries that drive cell intercalation throughout Drosophila axis extension

  1. Robert J Tetley
  2. Guy B Blanchard
  3. Alexander G Fletcher
  4. Richard J Adams
  5. Bénédicte Sanson  Is a corresponding author
  1. University of Cambridge, United Kingdom
  2. University of Sheffield, United Kingdom
Research Article
  • Cited 30
  • Views 2,389
  • Annotations
Cite this article as: eLife 2016;5:e12094 doi: 10.7554/eLife.12094

Abstract

Convergence and extension movements elongate tissues during development. Drosophila germ-band extension (GBE) is one example, which requires active cell rearrangements driven by Myosin II planar polarisation. A combinatorial code of Toll receptors downstream of pair-rule genes contributes to this polarization via local cell-cell interactions. We developed novel computational methods to analyse the spatiotemporal dynamics of Myosin II. We show that initial Myosin II bipolar cell polarization gives way to unipolar enrichment at parasegmental boundaries and two further boundaries within each parasegment, concomitant with a doubling of cell number as the tissue elongates. These boundaries are the primary sites of cell intercalation, behaving as mechanical barriers and providing a mechanism for how cells remain ordered during GBE. Enrichment at parasegment boundaries during GBE is independent of Wingless signaling, suggesting pair-rule gene control. We propose an updated cell-cell interaction model for Myosin II polarization that we tested in a vertex-based simulation.

Article and author information

Author details

  1. Robert J Tetley

    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  2. Guy B Blanchard

    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  3. Alexander G Fletcher

    School of Mathematics and Statistics, University of Sheffield, Sheffield, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  4. Richard J Adams

    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  5. Bénédicte Sanson

    Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom
    For correspondence
    bs251@cam.ac.uk
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Ewa Paluch, University College London, United Kingdom

Publication history

  1. Received: October 6, 2015
  2. Accepted: May 10, 2016
  3. Accepted Manuscript published: May 16, 2016 (version 1)
  4. Accepted Manuscript updated: May 23, 2016 (version 2)
  5. Version of Record published: June 21, 2016 (version 3)

Copyright

© 2016, Tetley et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 2,389
    Page views
  • 633
    Downloads
  • 30
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease
    Michael R McLaren et al.
    Research Article
    1. Computational and Systems Biology
    2. Immunology and Inflammation
    Kristian Davidsen et al.
    Tools and Resources