Eighteenth century Yersinia pestis genomes reveal the long-term persistence of an historical plague focus
- University of Tübingen, Germany
- Max Planck Institute for the Science of Human History, Germany
- Northern Arizona University, United States
- McMaster University, Canada
- The University of Sydney, Australia
- Université Bordeaux, France
Figures
Figure 1
Photograph of rescue excavations at l’Observance in Marseille, France.
LAPMO Université de Provence – URA 164 CNRS/AFAN.
Figure 2
Coverage plots for all reconstructed Observance core genomes.
Inner ring: GC content from low (brown, <30%) to high (orange, >55%). Outer concentric rings: from inner to outer, coverage plots of OBS107, 110, 116, 124, and 137 on a logarithmic scale. Axes are …
Figure 3
Phylogenetic tree for Yersinia pestis.
(A) Maximum likelihood phylogeny of Y. pestis genomic SNPs showing the position of the Observance (OBS) lineage (red) relative to those of 130 modern (black) and three ancient strains (Black Death …
Tables
Table 1
Quantitative PCR data for the pla gene obtained for the 19 Observance teeth extracted. Values below std curve detection are highlighted in grey.
| PCR Blks: | Copies per μl |
|---|---|
| PCR Blk 1 | 0 |
| PCR Blk 2 | 0 |
| PCR Blk 3 | 0 |
| PCR Blk 4 | 0 |
| Extraction Blks: | Copies per μl |
| BLk 1 | 0 |
| Blk 2 | 0 |
| Blk 3 | 0 |
| Blk 4 | 0 |
| Extracts: | |
| Sample | Copies per μl |
| 101a | 0 |
| 101b | 0 |
| 102 | 0 |
| 103 | 0 |
| 107 | 39 |
| 108 | 0 |
| 109 | 0 |
| 110 | 50 |
| 116 | 105 |
| 117a | 0 |
| 117b | 1 |
| 118 | 0 |
| 123 | 0 |
| 124 | 142 |
| 125 | 0 |
| 126 | 0 |
| 130 | 0 |
| 134 | 1 |
| 137 | 30 |
| 144 | 0 |
Table 2
Mapping statistics.
| Sample ID | Raw read pairs | Preprocessed reads | Mapped reads | % mapped | Dedupped | Duplication factor | Fold coverage | % covered |
|---|---|---|---|---|---|---|---|---|
| OBS107 | 14,646,710 | 15,395,287 | 1,850,734 | 12.02% | 851,096 | 2.17 | 12.20 | 84.79 |
| OBS110 | 9,879,257 | 10,458,571 | 1,581,158 | 15.12% | 855,988 | 1.85 | 14.61 | 90.63 |
| OBS116 | 16,858,273 | 17,876,883 | 2,931,554 | 16.40% | 1,363,415 | 2.15 | 20.04 | 92.56 |
| OBS124 | 48,797,602 | 52,345,884 | 4,972,347 | 9.50% | 973,763 | 5.11 | 13.34 | 85.37 |
| OBS137 | 120,018,142 | 129,095,434 | 12,669,625 | 9.81% | 1,583,596 | 8.00 | 24.40 | 92.76 |
Additional files
-
Supplementary file 1
Combined SNP table from three SNP calling approaches.
- https://doi.org/10.7554/eLife.12994.008
-
Supplementary file 2
List of genomes used in SNP calling and phylogeny.
- https://doi.org/10.7554/eLife.12994.009
