α-Bcd antibody detects Bcd protein in nuclear cycle 1 embryos (A) and in unfertilized eggs (B). Stage 14 oocytes (stained with DAPI) are covered with follicle cells initially and the dorsal appendages are juxtaposed (stage 14a; (C)); the nuclei of the follicle cells elongate and the follicle cells migrate anteriorly (stage 14b; (D)), form an anterior ring stage 14c; (E) that extrudes distally from the dorsal appendages, which separate from each other stage 14d; (F). High magnification images of DAPI-stained nuclei in (C,D,F) show the oocytes to be at prophase (14a) and true metaphase (14b, 14d) (Gilliland et al., 2009). In situ hybridization detects bcd RNA at all stage 14 oocytes (G,I,K); antibody staining detects Bcd protein in stage 14d oocytes (L) but not in younger stages (H,J). Orientation anterior left.